GlyNAC

JOHN 

I agree, they got extremely interesting results. But without oxidation we do not get the ROS that at least some senolytic substances rely on to remove senescent cells. Below an easy read that explains what we have to consider before taking huge amount of Acetyl cysteine.

N-Acetyl Cysteine: A Warning Shot | In the Pipeline (sciencemag.org)

Staffan Olsson This article you posted and the one about rats and lung tumors goes against a ton of other articles out there that shows NAC inhibits cancer growth:

Exactly the opposite of what your article claims. 

Please check the real data done in humans, here are only some studies:

https://www.researchgate.net/publication/47699443_Suppression_of_human_prostate_cancer_PC-3_cell_growth_by_N-acetylcysteine_involves_over-expression_of_Cyr61

https://www.researchgate.net/publication/330122763_N-acetylcysteine_decreases_malignant_characteristics_of_glioblastoma_cells_by_inhibiting_Notch2_signaling

http://www.ijcem.com/files/ijcem0096364.pdf

https://onlinelibrary.wiley.com/doi/full/10.1002/1097-0215(20001201)88:5%3C702::AID-IJC4%3E3.0.CO;2-3

And there are many more. Thoughts anyone?  This is why I need to talk to Dr. Green.  He is brilliant at weeding out the nonsense.  

Another thing to consider is the lab rats used in the study you mentioned above received astronomical doses of NAC.  Equivalent of a 75kg man taking around 75g NAC daily and the author of that article had worked at a number of pharmaceutical companies.  That doesn't' mean he is biased but combing through all these studies and articles one should take a lot of it with a grain of salt.  

Another study here for the benefits of NAC against cancer:

https://www.sciencedaily.com/releases/2017/11/171120141534.htm

JOHN The human biology is extremely complex and we seldom get any clear answers. the results from GlyNac are mazing. So I will probably do a limited self experiment for 3-4 weeks. But also try to make an informed decision regarding long term use based on how the “numbers# fall out in the  risk-reward equation.

Will you be using the high doses they used in the study? Let me know how you feel.  If you don't mind me asking how old are you? 

JOHN Yes, I will use high doses and report my experience here. I am not used to converting mmol/kg/day to mg/day so I have to make sure I get the numbers right. And I recently added another intervention to my protocol, so I must wait at least a couple of weeks before adding a new. 

Per the papers, the daily intake of each supplement is: ~100 mg/kg for glycine (~6 grams for a 60kg human) and ~130 mg/kg for N-acetylcysteine (~8 grams for a 60kg human), split into two doses.

Staffan Olsson did you end up doing this experiment? How'd it go?

J Oh A late reply, but here it is.

I bought everything (500 gr of each substance). But I decided against the above mentioned protocoll. So I am not using NACand I did not do the experiment. Now I only use  the glycine (approx 2-3 gr /day) for other reasons than the above mentioned GlyNac protocoll. 

Staffan Olsson thanks. Any particular reason you decided against the protocol? 

J Oh I am not sure how to implement  the GlyNac in my personal anti aging strategy  Oxidation is an important part of the signaling in the cell.  And this strategy might be disrupting important intracellular signaling.  But yet again, the approach looks very promising. I wait for more data.

Staffan Olsson did you see the newest study? Increased mice lifespan by 24%. I think it prob makes sense once we get older 

J Oh Yes I saw that study. And it looks very promising.

Staffan Olsson J Oh

My thinking is in this case much in line with Dr Brad's. 

https://www.youtube.com/watch?v=f7f1oaVtE0M&ab_channel=DrBradStanfield

Btw I like his videos. They are usually only around 10 minutes and not to speculative. 

Andrew Excellent questions!  I'm surprised that you felt a surge of energy, since glycine is a known somnorific.  The devil must be either in the high NAC dosage or some confounding variable.  I take 3g each of glycine and NAC at night and usually sleep rather well. 

Brian Valerie since that post I've found that the Thorne labs NAC does not contain stearate and I don't have the stomach issues with it that I previously had.  The NAC / Glycine combo as I understand it is 9g / 7g respectively.  I've been trying to follow that and take it during the day.  Have you tried using the higher dose either during the day or at night?  

Andrew Glad you found some NAC that agrees with you.  No, I haven't taken more than 3g.  Since more human trials are no doubt soon forthcoming, I'd rather be cautious about the higher doses.  As with almost all of my supplements, I even regularly abstain for a day or more in the hope of diminishing any potential homeostatic response.   Of course, even 3g would until very recently have been considered a high dose!  Do you think that there may be a benefit to taking it during the day?  It may be at least partially a placebo effect, but I believe that by taking it at night the glycine does help me sleep better.

Brian Valerie Before starting this experiment I had previously always taken 600mg NAC prior to bed.  My sleep has been on / off lately.  Perhaps what I'll do is split it up with 6g Nac during the day and 3g at night and see how that goes.  I do think there is a benefit during the day - when I take it consistently my overall energy levels are higher - and I like what the study said about grip strength considering I'm working out with X3 Bar (variable resistance bands).  Glad to hear it's working for you the way you've been taking it.  

Charles Richardson I appreciate your response. 

In terms of N Acetyl Cysteine (NAC)- the question is, is it the correct way to raise endogenous glutathione production or should it be increased through SKN-1 activation (biosynthesis) in which NAC has shown to decrease as in itself is an antioxidant. Some research suggests negative associations with NAC supplementation. 

https://www.nature.com/articles/s41467-021-24634-3

Of course I think we need enough NAC through diet and supplementation to provide sufficient amino acids, but what about the doses specified in the NAC/GLY study which had amazing results. 

Geoff Welch Yeah, I'm not that enamored with those amounts. That seems to me to be a pharmaceutical dosage. On the other hand, NAC has other functions such as protecting the lungs. But I'm not jumping on the bandwagon to take as much as was in that study.