GlyNAC

Has anyone tried Glycine/N-acetylcysteine?  It appears to have amazing effects on age reversal. 

Glycine and N‐acetylcysteine (GlyNAC) supplementation in older adults improves glutathione deficiency, oxidative stress, mitochondrial dysfunction, inflammation, insulin resistance, endothelial dysfunction, genotoxicity, muscle strength, and cognition: Results of a pilot clinical trial
https://onlinelibrary.wiley.com/doi/epdf/10.1002/ctm2.372

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    • JOHN
    • JOHN.1
    • 2 yrs ago
    • Reported - view

    I will need to get input from Dr Green on this one. I have been reading up on the subject and while this study looks promising I think it's too soon to jump the gun on taking large amounts of these 2 amino acids.  There's a possible link to cancer that scares me.  This study doesn't measure cancer risk over the long term use. 

    https://df6sxcketz7bb.cloudfront.net/manuscripts/127000/127647/jci.insight.127647.v1.pdf

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    • JOHN 

      I agree, they got extremely interesting results. But without oxidation we do not get the ROS that at least some senolytic substances rely on to remove senescent cells. Below an easy read that explains what we have to consider before taking huge amount of Acetyl cysteine.

       

      N-Acetyl Cysteine: A Warning Shot | In the Pipeline (sciencemag.org)

      Like 1
      • JOHN
      • JOHN.1
      • 2 yrs ago
      • Reported - view

      Staffan Olsson This article you posted and the one about rats and lung tumors goes against a ton of other articles out there that shows NAC inhibits cancer growth:

      Exactly the opposite of what your article claims. 

      Please check the real data done in humans, here are only some studies:

      https://www.researchgate.net/publication/47699443_Suppression_of_human_prostate_cancer_PC-3_cell_growth_by_N-acetylcysteine_involves_over-expression_of_Cyr61

      https://www.researchgate.net/publication/330122763_N-acetylcysteine_decreases_malignant_characteristics_of_glioblastoma_cells_by_inhibiting_Notch2_signaling

      http://www.ijcem.com/files/ijcem0096364.pdf

      https://onlinelibrary.wiley.com/doi/full/10.1002/1097-0215(20001201)88:5%3C702::AID-IJC4%3E3.0.CO;2-3

      And there are many more. Thoughts anyone?  This is why I need to talk to Dr. Green.  He is brilliant at weeding out the nonsense.  

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      • JOHN
      • JOHN.1
      • 2 yrs ago
      • Reported - view

      Another thing to consider is the lab rats used in the study you mentioned above received astronomical doses of NAC.  Equivalent of a 75kg man taking around 75g NAC daily and the author of that article had worked at a number of pharmaceutical companies.  That doesn't' mean he is biased but combing through all these studies and articles one should take a lot of it with a grain of salt.  

      Another study here for the benefits of NAC against cancer:

      https://www.sciencedaily.com/releases/2017/11/171120141534.htm

      Like 1
    • JOHN The human biology is extremely complex and we seldom get any clear answers. the results from GlyNac are mazing. So I will probably do a limited self experiment for 3-4 weeks. But also try to make an informed decision regarding long term use based on how the “numbers# fall out in the  risk-reward equation.

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      • JOHN
      • JOHN.1
      • 2 yrs ago
      • Reported - view

      Will you be using the high doses they used in the study? Let me know how you feel.  If you don't mind me asking how old are you? 

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    • JOHN Yes, I will use high doses and report my experience here. I am not used to converting mmol/kg/day to mg/day so I have to make sure I get the numbers right. And I recently added another intervention to my protocol, so I must wait at least a couple of weeks before adding a new. 

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      • JOHN
      • JOHN.1
      • 2 yrs ago
      • Reported - view

      Per the papers, the daily intake of each supplement is: ~100 mg/kg for glycine (~6 grams for a 60kg human) and ~130 mg/kg for N-acetylcysteine (~8 grams for a 60kg human), split into two doses.

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      • J Oh
      • J_Oh
      • 2 yrs ago
      • Reported - view

      Staffan Olsson did you end up doing this experiment? How'd it go?

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    • J Oh A late reply, but here it is.

       

      I bought everything (500 gr of each substance). But I decided against the above mentioned protocoll. So I am not using NACand I did not do the experiment. Now I only use  the glycine (approx 2-3 gr /day) for other reasons than the above mentioned GlyNac protocoll. 

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      • J Oh
      • J_Oh
      • 2 yrs ago
      • Reported - view

      Staffan Olsson thanks. Any particular reason you decided against the protocol? 

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    • J Oh I am not sure how to implement  the GlyNac in my personal anti aging strategy  Oxidation is an important part of the signaling in the cell.  And this strategy might be disrupting important intracellular signaling.  But yet again, the approach looks very promising. I wait for more data.

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      • J Oh
      • J_Oh
      • 2 yrs ago
      • Reported - view

      Staffan Olsson did you see the newest study? Increased mice lifespan by 24%. I think it prob makes sense once we get older 

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    • J Oh Yes I saw that study. And it looks very promising.

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    • Staffan Olsson J Oh

      My thinking is in this case much in line with Dr Brad's. 

      https://www.youtube.com/watch?v=f7f1oaVtE0M&ab_channel=DrBradStanfield

      Btw I like his videos. They are usually only around 10 minutes and not to speculative. 

      Like 1
    • Andrew
    • Andrew_F
    • 2 yrs ago
    • Reported - view

    When are you taking it?  How many days thus far?  I've only tried the 9g / 7g dose once.  I had previously taken NAC and Glycine at night before bed, so that's when I tried it.  Apparently bad idea - as I had a surge of energy and sleep was disturbed.  Also - with the brands of NAC and Glycine I purchased (Vitacost and DoubleWood respectively) I noticed after the fact that they contain Magnesium Stearate in them, so at these high doses I'm wondering if the latent sluggish feeling I had for the next 24 hours was due to that.  

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      • Brian Valerie
      • Semi-Retired Health Education Teacher
      • Brian_Valerie
      • 2 yrs ago
      • Reported - view

      Andrew Excellent questions!  I'm surprised that you felt a surge of energy, since glycine is a known somnorific.  The devil must be either in the high NAC dosage or some confounding variable.  I take 3g each of glycine and NAC at night and usually sleep rather well. 

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      • Andrew
      • Andrew_F
      • 2 yrs ago
      • Reported - view

      Brian Valerie since that post I've found that the Thorne labs NAC does not contain stearate and I don't have the stomach issues with it that I previously had.  The NAC / Glycine combo as I understand it is 9g / 7g respectively.  I've been trying to follow that and take it during the day.  Have you tried using the higher dose either during the day or at night?  

      Like 1
      • Brian Valerie
      • Semi-Retired Health Education Teacher
      • Brian_Valerie
      • 2 yrs ago
      • Reported - view

      Andrew Glad you found some NAC that agrees with you.  No, I haven't taken more than 3g.  Since more human trials are no doubt soon forthcoming, I'd rather be cautious about the higher doses.  As with almost all of my supplements, I even regularly abstain for a day or more in the hope of diminishing any potential homeostatic response.   Of course, even 3g would until very recently have been considered a high dose!  Do you think that there may be a benefit to taking it during the day?  It may be at least partially a placebo effect, but I believe that by taking it at night the glycine does help me sleep better.

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      • Andrew
      • Andrew_F
      • 2 yrs ago
      • Reported - view

      Brian Valerie Before starting this experiment I had previously always taken 600mg NAC prior to bed.  My sleep has been on / off lately.  Perhaps what I'll do is split it up with 6g Nac during the day and 3g at night and see how that goes.  I do think there is a benefit during the day - when I take it consistently my overall energy levels are higher - and I like what the study said about grip strength considering I'm working out with X3 Bar (variable resistance bands).  Glad to hear it's working for you the way you've been taking it.  

      Like 1
  • Just FYI, NAC is also good to take before consuming alcoholic beverages. It helps the liver clear the bad byproducts. I do that regularly and definitely fewer bad side effects.

    Like 1
  • Hey guys, 

    I'm hoping someone can answer the question of antioxidants, especially at high doses, causing more harm than good by decreasing and preventing beneficial signalling of ROS. What measurable impact does N Acetyl Cysteine have on hormesis? 

    It seems as though antioxidants follow a U shaped curve, too little being just as bad as two much, but I'm not sure a stress response "hormesis" > NRF2/SKN1 is the answer to solving the complexities of oxidative stress. 

    Any insight would be appreciated. 

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  • NAC provides the raw materials for your body to make glutathione which a powerful internal antioxidant. You're right in that exogenous antioxidants have not turned out to be a good thing.

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    • Charles Richardson I appreciate your response. 

      In terms of N Acetyl Cysteine (NAC)- the question is, is it the correct way to raise endogenous glutathione production or should it be increased through SKN-1 activation (biosynthesis) in which NAC has shown to decrease as in itself is an antioxidant. Some research suggests negative associations with NAC supplementation. 

      https://www.nature.com/articles/s41467-021-24634-3

      Of course I think we need enough NAC through diet and supplementation to provide sufficient amino acids, but what about the doses specified in the NAC/GLY study which had amazing results. 

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    • Geoff Welch Yeah, I'm not that enamored with those amounts. That seems to me to be a pharmaceutical dosage. On the other hand, NAC has other functions such as protecting the lungs. But I'm not jumping on the bandwagon to take as much as was in that study.

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