GlyNAC

Has anyone tried Glycine/N-acetylcysteine?  It appears to have amazing effects on age reversal. 

Glycine and N‐acetylcysteine (GlyNAC) supplementation in older adults improves glutathione deficiency, oxidative stress, mitochondrial dysfunction, inflammation, insulin resistance, endothelial dysfunction, genotoxicity, muscle strength, and cognition: Results of a pilot clinical trial
https://onlinelibrary.wiley.com/doi/epdf/10.1002/ctm2.372

46replies Oldest first
  • Oldest first
  • Newest first
  • Active threads
  • Popular
  • That's very interesting.  The only source that I see online seems to be Celltrient.  They sell 112 x 600 mg + 600 gm caps, taken 2/day, for $60.79.  However, I see that Life Extension sells Glycine ($9.00 for 100 x 1000 mg caps) and N‐acetylcysteine ($10.50 for 60 x 600 mg caps) as separate supplements.  I think that works out to be around 1/2 the Celltrident price.

        Perhaps it's worth it, but I think I'll wait for others to report any age reversal observations after taking this supplement.   It would be nice if someone would determine their Horvath DNAm age before and after taking a month or so of GlyNAC supplements.

    Like
      • aribadabar
      • aribadabar
      • 6 mths ago
      • 1
      • Reported - view

      JGC I don't see the point of overpaying for a designer product if we are talking about simple amino acids.

      Just buying their Ajipure version is sufficient if one is concerned about quality.

       

      What I am more concerned about is the large NAC dose which is reported to cause PAH.

       

      Personally, I would stick to max 1800mg /d NAC and 5g/d Glycine and call it a day.

      If it helps with boosting GSH to youthful levels, good. If it's insufficient, it is still better than nothing and I am fine with that.

      Like 1
      • Qimin You
      • Qimin_You
      • 6 mths ago
      • Reported - view

      aribadabar Your concern is that PAH causes cancer?  Will NAC be transformed to PAH in the body? Do you have a reference?

      Like
      • aribadabar
      • aribadabar
      • 6 mths ago
      • Reported - view

      Qimin You PAH= Pulmonary arterial hypertension.

      Like
      • Qimin You
      • Qimin_You
      • 6 mths ago
      • Reported - view

      aribadabar OK, I thought it was Polycyclic Aromatic Hydrocarbon 

      Like
  • The study quoted above indicates that the GlyNAC benefits go away when the supplement is halted.  That suggests that no epigenetic reprogramming has been achieved.

    Like
      • Qimin You
      • Qimin_You
      • 6 mths ago
      • 1
      • Reported - view

      JGC I am 55kg, so I should take abut 5g/day.  But I think it will be safer to take less, say 2g of each to start with.  there is not much data about GlyNAC help epigenetic reprogramming.  I  searched MEDPUB, and found all related publications are from the same Lab. So...

      But I think at least they shall be safe, they are only amino acids...

      Like 1
  •     I just calculated the dosage of the GlyNAC supplements that was used in the Baylor Med study.  They say that they administered glycine (1.33 mmol/kg/day) and cysteine (0.81 mmol/kg/day).  That means they would give a subject weighing 70 kg about 7,000 mg/day  (7 g/day) of both Glycine and N-acetylcysteine.  That would mean one would have to take a lot of caps per day.  The dosage recommended on the Celltrident bottle is only 1.2 g/day.  One would go through a "$60.79 - 1 month supply" on about 5 days.

    Like 1
  • Does anyone know any details of the timing of the dosage?  All before bed?  Spread out over the day?  I've been taking NAC (600mg) before bed for a while.  Going to 7g or 9g seems like a lot.  I might schedule a follow up with Dr. Green - just to discuss this.  

    Like 1
      • JGC
      • JGC
      • 6 mths ago
      • 2
      • Reported - view

      Andrew 

          The Baylor Med paper says that the 8 old (71-80) and the 8 young (21-30) participants were given prepared capsules containing the GlyNAC dosages, and they were checked periodically to make sure they were taking them.  There is no information in the paper about when they were told to take them (if they were) but it sounds like they took one big dose (in the capsule) at a time of their own choosing.

          Following Aribadabar's suggestion, I searched on Aijpure and found that Swanson has Glycine for $2.99 for 60  x 500 mg caps and N‐acetylcysteine for $6.39 for 100 x 600 mg caps.  That's rather better than the LE prices.

      Like 2
      • JGC
      • JGC
      • 6 mths ago
      • 1
      • Reported - view

      Staffan Olsson 

      BulkSupplements.com also sells NAC powder, but it's a bit more expensive: $50/kg.

      Like 1
    • Andrew I’m a patient of Dr. Green as well. I just emailed him the same question. Did he get back to you. Is it to be taken everyday at 7 and 9 grams?

      Like
      • Andrew
      • Andrew_F
      • 6 mths ago
      • Reported - view

      Benjamin Schoolman  - in response to my questions regarding senolytics and GlyNac - he replied "If you would like to discuss this in further detail, please schedule a consultation"

      Like
      • aribadabar
      • aribadabar
      • 4 mths ago
      • Reported - view

      JGC There is a bulk powder version of Ajipure Glycine too. 

      Like
      • aribadabar
      • aribadabar
      • 4 mths ago
      • Reported - view

      JGC Also, your link points to the non-Ajipure NAC offering. The correct one is a more expensive but not terribly more so.

      Like
  • Why all this capsule talk when you can buy bulk powder. Mix it with something into a drink. I haven't tried it but sounds like it might work.

    Like
      • aribadabar
      • aribadabar
      • 6 mths ago
      • 1
      • Reported - view

      Allen Glycine is very pleasant but cysteine isn't.  You need to overwhelm it with something sweet and dilute substantially.

      Like 1
      • Allen
      • Allen_Rosenberg
      • 6 mths ago
      • Reported - view

      aribadabar Thanks for the info. I have nac capsules and opened one up after reading your post. You're right, it's really strong. I had no idea it was that bad. 

      Like
    • yikes, just tried a gram of NAC powder in my shake and taste is incredibly bad.Not sure how to overwhelm it to get it down

      Like
      • Fred Cloud
      • Fred_Cloud
      • 6 mths ago
      • Reported - view

      Paul Beauchemin Yeah, its nasty. I just stick with capsules. You can get a big bottle pretty cheap on amazon.

      Like
  • These dosages seem very high. However, as Dr Green points out below it's the amount that is found in a large steak.  I calculated for me as a 90 kg male it would cost me about $75 dollars per month to take this large dose of both supplements.  Hopefully Dr. Green has his compounding pharmacy on the case. My wife has her annual appointment with Dr Green next week so she will discuss the new protocol.  Dr Green's website talks about adding this regimen to his anti-aging protocol.  I already take 600mg/day of the NAC.  I may add the glycine to this in the morning on an empty stomach.  Taking amino acids on an empty stomach is the preferred method. See from Dr Green's website below:

    In March 2021, high dose supplementation with amino acids cysteine as NAC and glycine was revealed as an  effective treatment for mitochondrial dysfunction and that is now also included in protocol. 

    Treatment of Aging

    A recent paper from Baylor College of Medicine, Houston, March 2021 represents the first great breakthrough in the treatment of aging itself. In 1956 Harman presented his free radical theory of aging. Just 67 years later, that theory has borne fruit. Mitochondria are little batteries that generate chemical energy that provide the energy for life. During the process, mitochondria generate toxic reactive oxygen species (ROS) which cause harmful oxidative stress to mitochondria. The major antioxidant mitochondia depend on for protection against the damaging effects of oxidative stress is GLUTATHIONE. The problem is in older persons, Glutathione levels are at too low a level for protection. This results in mitochondrial dysfunction. Glutathione is composed of three amino acids: glutamic acid, cysteine, and glycine. In older persons the levels of cysteine and glycine are too low to form Glutathione (GSH). The solution to the problem is mind-blowingly simple. Just supply an adequate amount of CYSTEINE AND GLYCINE. The lead researcher, Rajagopal Sekhar, who first published a similar study in 2011; did just that and the results were EXTRAORDINARY.  The dose for a 70 kilo man was 9 gram of cystine and 7 grams of glycine (about the amount of these two amino acids in 1.5 pounds of steak). 

    The results on mitochondrial function, oxidative stress, inflammation, insulin resistance, walking speed, grip strength, cognitive function and other markers of aging dysfunction were remarkable. In a prior study excellent results were seen at 2 weeks. In this study persons were tested at 12 and 24 weeks. By 24 weeks, older persons were showing similar results to young persons in critical areas. Upon stopping treatment, all benefits were gradually lost. 

     

    What was different is this study: BOTH Glycine and cysteine (as N-acetyl cysteine (NAC) were administered and in much higher doses.  

     

    "Glycine and N-acetylcysteine (GlyNAC) supplementation in older adults improves glutathione deficiency, oxidative stree, mitochondrial dysfunction, inflammation, insulin resistance, endothelial dysfunction, genotoxicity, muscle strength, and cognition: Results of a pilot clinical trial"; Kumar, Sekhar, 2021.    

    Like 2
  • I would also assume that there were other improvements in the subjects that were not mentioned or yet known. The study was limited by what it could test. Maybe there were improvements in hearing, sight, reaction time, cardiovascular function(ejection fraction), or sleep to name a few. 

    Like 2
  • I will need to get input from Dr Green on this one. I have been reading up on the subject and while this study looks promising I think it's too soon to jump the gun on taking large amounts of these 2 amino acids.  There's a possible link to cancer that scares me.  This study doesn't measure cancer risk over the long term use. 

    https://df6sxcketz7bb.cloudfront.net/manuscripts/127000/127647/jci.insight.127647.v1.pdf

    Like
  • When are you taking it?  How many days thus far?  I've only tried the 9g / 7g dose once.  I had previously taken NAC and Glycine at night before bed, so that's when I tried it.  Apparently bad idea - as I had a surge of energy and sleep was disturbed.  Also - with the brands of NAC and Glycine I purchased (Vitacost and DoubleWood respectively) I noticed after the fact that they contain Magnesium Stearate in them, so at these high doses I'm wondering if the latent sluggish feeling I had for the next 24 hours was due to that.  

    Like 1
      • Brian Valerie
      • Semi-Retired Health Education Teacher
      • Brian_Valerie
      • 3 mths ago
      • 1
      • Reported - view

      Andrew Excellent questions!  I'm surprised that you felt a surge of energy, since glycine is a known somnorific.  The devil must be either in the high NAC dosage or some confounding variable.  I take 3g each of glycine and NAC at night and usually sleep rather well. 

      Like 1
      • Andrew
      • Andrew_F
      • 3 mths ago
      • 1
      • Reported - view

      Brian Valerie since that post I've found that the Thorne labs NAC does not contain stearate and I don't have the stomach issues with it that I previously had.  The NAC / Glycine combo as I understand it is 9g / 7g respectively.  I've been trying to follow that and take it during the day.  Have you tried using the higher dose either during the day or at night?  

      Like 1
      • Brian Valerie
      • Semi-Retired Health Education Teacher
      • Brian_Valerie
      • 3 mths ago
      • 2
      • Reported - view

      Andrew Glad you found some NAC that agrees with you.  No, I haven't taken more than 3g.  Since more human trials are no doubt soon forthcoming, I'd rather be cautious about the higher doses.  As with almost all of my supplements, I even regularly abstain for a day or more in the hope of diminishing any potential homeostatic response.   Of course, even 3g would until very recently have been considered a high dose!  Do you think that there may be a benefit to taking it during the day?  It may be at least partially a placebo effect, but I believe that by taking it at night the glycine does help me sleep better.

      Like 2
      • Andrew
      • Andrew_F
      • 3 mths ago
      • 1
      • Reported - view

      Brian Valerie Before starting this experiment I had previously always taken 600mg NAC prior to bed.  My sleep has been on / off lately.  Perhaps what I'll do is split it up with 6g Nac during the day and 3g at night and see how that goes.  I do think there is a benefit during the day - when I take it consistently my overall energy levels are higher - and I like what the study said about grip strength considering I'm working out with X3 Bar (variable resistance bands).  Glad to hear it's working for you the way you've been taking it.  

      Like 1
  • Just FYI, NAC is also good to take before consuming alcoholic beverages. It helps the liver clear the bad byproducts. I do that regularly and definitely fewer bad side effects.

    Like
  • Hey guys, 

    I'm hoping someone can answer the question of antioxidants, especially at high doses, causing more harm than good by decreasing and preventing beneficial signalling of ROS. What measurable impact does N Acetyl Cysteine have on hormesis? 

    It seems as though antioxidants follow a U shaped curve, too little being just as bad as two much, but I'm not sure a stress response "hormesis" > NRF2/SKN1 is the answer to solving the complexities of oxidative stress. 

    Any insight would be appreciated. 

    Like
  • NAC provides the raw materials for your body to make glutathione which a powerful internal antioxidant. You're right in that exogenous antioxidants have not turned out to be a good thing.

    Like 1
    • Charles Richardson I appreciate your response. 

      In terms of N Acetyl Cysteine (NAC)- the question is, is it the correct way to raise endogenous glutathione production or should it be increased through SKN-1 activation (biosynthesis) in which NAC has shown to decrease as in itself is an antioxidant. Some research suggests negative associations with NAC supplementation. 

      https://www.nature.com/articles/s41467-021-24634-3

      Of course I think we need enough NAC through diet and supplementation to provide sufficient amino acids, but what about the doses specified in the NAC/GLY study which had amazing results. 

      Like
    • Geoff Welch Yeah, I'm not that enamored with those amounts. That seems to me to be a pharmaceutical dosage. On the other hand, NAC has other functions such as protecting the lungs. But I'm not jumping on the bandwagon to take as much as was in that study.

      Like
  • I’m wondering why it wouldn’t be easier just to supplement with Glutathione?

    Like
    • Curt Lizzi  Glutathione does not absorb well through the digestive system from the research I've seen.

      Like
    • Charles Richardson I thought that might be the reason. Also wondering why they only used 2 of the 3 AAs that make up Glutathione?

      this would be a protocol that would be hard to follow long term just because of the volume!

      Like
    • Curt Lizzi I think it would be hard to be deficient in glutamic acid (that's my understanding).

      Like
    • Charles Richardson They used to think that but current research has debunked it and apparently it is absorbed which is why they started selling it as a supplement now. Now having said that, it may pass the first obstacle in the gut, but it may only enter circulation and not penetrate the cell membrane. But still therapeutic for the liver etc.

      Like
Like2 Follow
  • 2 Likes
  • 3 wk agoLast active
  • 46Replies
  • 791Views
  • 19 Following