JGC That sounds like a good protocol. I’ve read about bioperine from LEF. I don’t have access to Dasatinib yet but I did take Fisetin, Quercetin, Resveratrol, with some milk thistle and coconut oil for the first three days of a 5 day fast recently. I did also take some piperlongumine as well. I thought I might get a better effect while fasting. Thanks so much for the tip. I’m going to give it a try.

Fred Cloud 

One way to interpret information about AKG-rejuvant is that epigenetic age can be reversed in some tissues and in some organs and that the tissue specific rejuvenation and the reduction of epigenetic age of tissues and organs does not translate into a reduction of the epigenetic age of the whole organism.  In other words, the reversed epigenetic age of tissues does not correlate with a reversal of the epigenetic age of the person.

If AKG achieves a tissue specific rejuvenation that makes a human body healthier and less prone to the diseases and the frailty that comes with old age then the result is a healthier body but not a longer lifespan (or maybe only a modest gain in average lifespan).

AKG might compress morbidity and make some tissues/organs healthier and rejuvenated to a younger state. If the epigenetic clock rejuvant use “catch” some tissue specific rejuvenation then I know  what I can expect from AKG. A healthier body but not an expanded lifespan. 

Not all epigenetic clocks are the same, some are good at measuring the biological age of the human body. Others are better at measuring the biological age of organs/tissues and disease related phenotypes. I don’t know which clock Rejuvant used. As far as I have learned, Kennedy himself and rejuvant speaks more about increased healthspan and compressed morbidity than of increased lifespan.

Michael Lustgarten, made a short video about the pros and cons of different epigenetic clocks. Some clocks are better in measuring the general biological ageing vs chronological ageing, Other epigentic clocks are better at measuring disease related changes.

https://www.youtube.com/watch?v=iNnvF1Eh7VE

AKG is a key metabolite in the krebs cycle and by manipulating it we might delay age related diseases and some organ dysfunctions. If this is the case then I know what to expect from it and I keep AKG in my regimen. I guess the choice I have to make is between wait for more data and till then take a leap of faith based studies on mammals or wait for more data and till that arrives stay away from AKG. I stay with AKG for now.  

https://www.lifespan.io/news/a-summary-of-alpha-ketoglutarate/

Staffan Olsson 

    You wrote: "I don’t know which clock Rejuvant used."  The Rejuvant clock used only 13 methylation sites, while Horvath-based clocks use several hundred.  That suggests that the Revjuvant clock was "cherry-picked" to show evidence of epigenetic reprogramming, and that, as you suggest, actually only a few cell-types were affected.

    I note that in an N=1 self-experiment, jimmy2 did a before/after clock comparison involving taking the Rejuvant product for 6 months.  Using two different commercial DNAme clocks, he found no evidence of epigenetic reprogramming.

    The Rejuvant web site contains lots of misinformation about the DNA methylation process.  Whatever their internal expertise, I suspect that they have some snake-oil salesmen on their payroll, and I would regard any claims about general epigenetic reprogramming with suspicion.  However, the Buck Institute work does indicate some significant AMG benefits (at least for worms an mice), and I plan to add the Swanson version of AMG to my evening supplements  when my order arrives.

Staffan Olsson Various tissues could be rejuvenated at different rates and you would never know because the DNA tested and used for the popular blood based methylation age test like mydnage is only white blood cells.

So it doesnt look at the various tissues throughout the body. Sometimes it can be misleading when a test says they use 11 sites or 200 sites or thousands of sites, it isnt sites in various tissues in the body, it is sites on the dna of one type of cell, in this case white blood cell.

Trueme which is what rejuvant uses  is a saliva based test, so I assume they are using cells from your cheek but I would have to verify that.

Fred Cloud 

    I don't recall the venue (maybe ARDD?), but I do recall a talk by Steve Horvath in which he reported on the DNAm clock readings for various tissue samples from the same human individual.  I think he said that, in general, tissues age at about the same rate.  The exception was the tissue of women's breasts, which age faster than other tissue.   So it is possible to do differential tissue aging studies (but maybe only after an autopsy ☹️).

    I note that one can get DNAm tests done based on saliva or urine (as well as blood), so the those probably do not use white blood cells.

Robert Olin 

L-Ornithine a-Ketoglutarate (OKG)

https://www.bulksupplements.com/products/l-ornithine-a-ketoglutarate-okg-powder?_pos=1&_sid=7961ea6a4&_ss=r

JGC What is your rationale for taking AKG in the evening?

FWIW I take it first thing in the morning , on an empty stomach. Reason being, better absorption.

aribadabar Just heard that the calcium acts as a buffer to counter it's acidity ie taking on an empty stomach.