Recent Plasma-Replacement Results
Two recent results have been published that are worth reporting here and are recommended for careful reading.
The first of these is a paper by Dr. Harold Katcher, Dr. Steve Horvath, and others reporting on a development originated by Nugenics Research of Mumbai, India. The work was done on by applying a component of young plasma (given the name Elixir) extracted from the blood of young rats. This Elixir was administered to aging rats, and measurements of the Horvarth methylation bio-age clock were done before and after the treatment. The before and after clocks indicated that in blood, heart, and liver tissues the bio-age was reduced by a factor of two. Less pronounced but significant clock-reduction was observed in the hypothalamus.
The second paper is the work of Prof. Irina Conboy's group at UC Berkeley. They examined the question of whether young-blood-produced tissue regeneration comes from the presence of beneficial components in the young blood or from the absence or dilution of harmful components in the old blood. To do this, they replaced the plasma in the blood of aging mice with saline solution containing 5% purified albumin. Unfortunately, they did not do bio-clock measurement on the results, but they noted beneficial effects to muscle, heart, and nerve tissues equal-to or exceeding those of young-old plasma exchange. The implication is that the benefits of young blood may lie in the dilution of harmful components present in old blood.
These are both preliminary studies using animal models, but their implications for us aging humans who could use some rejuvenation are very interesting. The Conboy results are particularly of interest because immediate application to humans would probably not encounter FDA roadblocks. (FDA Experts: please comment!)
The closest you can replicate this now without a doc and machines would be to donate blood a couple of times a year and take gdf11 at home. The next level would be to get a doc with a plasmapheresis machine and have him replace some of your plasma and buy some plasma from the ambrosia company. Speaking of, I wonder if these plasma doctors like ambrosia will offer some sort of plasma exchange as an extra option.
Perhaps some docs will offer this as a standalone service much like the chelation docs back in the day would have rooms full of people in the IV room. The closest you can get to that now is In germany, they have lots of plasmapheresis treatment centers where they will filter your blood and should be able to replicate the conboy effect. Much like chelation requires frequent treatments to maintain, I wonder how often you would have to do dilute your plasma to maintain results?
I wonder if providers will pop up and start offering plasma dilution therapy based on the results of this study? Anybody can replicate this study therapy and it doesn't involve giving anything controversial. Just a plasmapheresis machine. Which leads me to my next point. How will the FDA look at this if doctors started offering it? They came down on Ambrosia for selling young plasma, but this therapy doesn't actually use other peoples plasma that they could take issue with. I see this study as a breakthrough, not just therapeutically but from a regulatory hurdle breakthrough to offer this to the masses right now.
So will providers popup and will the fda shut them down?
- if epigenetic clock reversal is proven in dilution experiment (seems probable if there is lasting effect to proteome) than there is another reason to believe in hyperfunction theory of aging
- hyperfunction theory is based on the assumption of post-reproductive detrimental developmental algorithms. If the epigenetic age may be shifted by means on of altered inter-cellular signalling we shall all consider that neither damage nor "loss of information" are suitable to describe the aging process.
- furthermore dilution of old blood shall show a positive effect even for normal plasma donation (without albumin compensation). Example of US regulation:Donor Weight>175lbs(80kg); max plasma collection volume=880ml; Max Plasma Loss/1 year = 83.2 Litres
- there are no elderly donors of plasma.. (due to regulation). That is probably one reason that there are no data to support the positive effects of old blood dilutions.
On the recent Conboy study (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288913/pdf/aging-12-103418.pdf ) replacing 50% plasma with saline and albumin, the conclusion appears to be that old detrimental "factors" are being removed allowing for multi-tissue regeneration. I've estimated that it takes about 10 months (6 cycles) to off-load 50% of my blood volume by donations (8% of blood volume each donation plus bi-monthly blood work). I have low ferritin, and blood donation causes major drop in iron stores, so I'm supplementing Fe and doing CBC and CMP blood tests every two months just prior to donations. Ferritin test less frequently. Third donation in 2 weeks. At 66 yo it's worth the experiment and the cost is minimal.
In this 2019 talk https://www.youtube.com/watch?v=5y2_H6d-6VU&t=364s
she states (at ~ 8 minutes) that it is not justified to interpret the parabiosis experiments as showing that there is some secret ingredient in young blood responsible for the positive effects. Plasma exchange (and parabiosis) appears to work for the older mice by resetting the interactions (signaling) between cells to younger levels.
Российские биохакеры повторили эксперимент по разведению плазмы у человека https://rlegroup.net/2020/12/06/realnyj-biohaking-chast-pervaja-plazmaferez/
Translated table <min_opt, max_opt, before, after, delta> from the russian text.
After = 3 days after neutral plasma exchange.
The Conboys replaced old plasma with saline at 5% Albumin. They concluded that it was the removal of plasma that was responible for the effect. How do we know that it was not the 5% Albumin? Here is the reasoning:
a) Albumin of 5% is near optimum. It is very likely that the old mice had a level much lower than that!
b) Several clocks (i.e. aging.ai), show that the level of Albumin is strongly inversele=y corelated with age.
So it would not be far fetched to assume that it was the 5% Albumin in saline that caused the improvements, by increasing the average content of albumin in the blood after treatment.
In my opinion, they should test for the albumin level before treatment, and inject saline with a content of albumin equal to the albumin level before treatment. Or, they could have some control old mice that they injected plain albumin, to make sure that it was not albumin that caused the effects.
On the other hand, Harold Katcher's experiment was robust, and the effects stunning. I can't wait to see how the elixir treatment translates to human beings. If it does even to some extent, I think we can forget about all other rejuvenation treatments . There will be no need for any of them for the next few decades.
Plasma Donation Info
As noted in prior posts I've restarted blood donations every 2 months, and just gave my 5th donation. I also received feedback from the donor center about plasma donation as they are currently looking for donors. They use a centrifugal machine to separate blood cells and platelets and send those back. The volume removed depends on certain parameters like weight and BMI, but appears to average a bit over 600 mls, Plasma comprises about 55% of whole blood volume and is present at approx. 43 ml per Kg of body weight. For me that would be a plasma volume of about 3300 mls. So one donation would offload about 20% of plasma. Enough to make a difference in the signaling milieu? Don't know. In addition, plasma only donation can be a done every 4 weeks, so in 8 weeks 3 donations are possible. This would reach the 50% threshold which mimics the Conboy mouse study. It's a free process which provides a benefit to people in need, and may help remove, at least temporarily, a fraction of proteins which promote aging through their signaling (i.e dial down the negative signals a bit). Side effects are potentially worse than normal blood donation, so if you do this educate yourself.
Fluctuates, but these are the results over the last 18 months when I did test for it:
34, 20, 45, 15, 37, 23
My last transferrin was 21%. As I've posted I believe elsewhere, iron dumping is one aspect of my anti-aging protocol.
"Ferritin is not a good indicator of iron stores. Serum ferritin is a test that was formerly thought to be directly related to the amount of tissue ferritin, and thus, something that could be used to measure the amount of stored iron, which was believed to always be stored bound to ferritin. Today, modern research has shown that actually, serum ferritin levels are not related to tissue ferritin levels, and that iron is only stored bound to ferritin in tissues that produce ferritin, like the liver, and is stored in free molecule form in other tissues, like cardiac muscle, that do not produce ferritin. Transferrin saturation is the indicator of excess iron storage, and needs to be below 40% in everyone, more especially those individuals who have HH. Why? Simple. When transferrin saturation is over 40%, which indicates that iron storage sites around the body have reached their normal capacity, two things happen. One, is the liver releases the HFE protein (starting when %TS is over 20%), which is supposed to bind with receptors in other cells, stimulating the release of hepcidin, which in turn binds with receptors in intestinal cells, halting the absorption and release into the blood stream of iron molecules. The second one is the reason that 40% was chosen as the threshold - at 40%, there's not enough transferrin to bind with all of the iron being secreted to the bloodstream by the intestinal cells, causing free iron molecules to circulate in the bloodstream, getting deposited wherever they can, where they act as free radicals, causing damage."
Anti-aging testing conference - ILA-HEALES - 11 February 2021
You can watch the session of Irina Conboy, Vera Gorbunova, Harold Katcher and Hanadie Yousef from here which are about blood proteins.
Also, could you give some feedback please, did you observe any benefit after blood or plasma donations? I mean cholesterol, blood pressure, liver and so on...