How to increase the bioavailability of Fisetin

Staffan Olsson 

That's an interesting article.  Near the end, it discusses techniques for the delivery of polyphenols, and mentions the use of cyclodextrins.  Apparently ß-cyclodextrin, the middle-size cyclodextrin shown below, works well for the delivery of at least some polyphenols.  It's a ring- or doughnut-shaped molecule that looks like this:

The polyphenol to be delivered fits in the hole in the middle of the ß-cyclodextrin.  The outside of the ring is hydrophylic and the center is hydrophobic, so it can dissolve otherwise insoluble molecules in water.  The air freshener Febreze uses ß-cyclodextrin as its active ingredient to trap bad-smelling molecules.   It is listed as non-toxic when taken orally.

Amazon sells ß-cyclodextrin for $22.95 for 113 grams.   I'm wondering if one should try combining it with our flavenoids of interest, Fisetin, Quercetin, Resveratrol, Curcumin, etc., to enhance their bioavailability. 

JOHN 

I'm a physicist, not an MD, so my opinion is not worth much in this area.  However, if it were me deciding, I'd do it, since the only known side effects of burst-doses of Fisetin and D+Q are mild flu-like symptoms that last a few hours.

More on Cyclodextrin

     A little further research indicates a problem in what I suggested.  Apparently, ß-cyclodextrin is about x10 less soluble in water than its molecular relatives alpha and gamma cyclodextrin because it is more tightly bound internally.  There are variants of ß-cyclodextrin that are more water soluble and thus better for promoting bio-availability, but these so not seem to be readily available for sale.

     Also, a pill company called Nature's Essentials markets a rather pricey spectrum of products, including curcumin, metatonin, vitamin-C, and resveratrol, that are encased in a liposome and then protected by cyclodextrin for greatly improved bio-availability.  It is not clear which variety of cyclodextrin is being used, but I suspect that it is alpha cyclodextrin.  I note that Amazon sells alpha-cyclodextrin powder at $18.00 for 5 grams, promoting it as a dietary fiber and fat blocker because it passes right through the digestive system without interaction.

JGC I have checked out nature's essentials line of products. I hesitate to try them until they have been tested by a third party. I sometimes check what https://www.consumerlab.com/ has to say about the content of supplements.  They  test supplements and post the results. As far as I can see they have never tested anything from nature's essentials. 

JGC Nature's Essentials is a great fine regardless whether they have Consumerlab testing or not.  Consumer Labs tests a fraction of what is available in the marketplace so it is not surprising that they have tested Nature's Essentials products.

David Hanson I agree they only test a fraction of what is available. But when learning about a new brand selling products in new formulations, then one want to have some proof of their concept. I hold nothing at all against this company. But I have in mind what some perons in this forum have experienced when it comes to rapamycin.  And I am happy to learn more about Nature's essentials and their products. Do you have any experience of nature's essentials line of product? 

Staffan Olsson No I haven't used Nature's Essentials (living in SE Asia for 5 months this winter prevents me from buying from t them) yet.  I did write to them this morning asking them for any third party testing.  One thing that they have going for them is that they have a corporate office in Atlanta.  I am not sure where their supplements are manufactured but at least the quality process is being managed out of the USA.  I think this is far better than buying from India or China.  Concerning the Rapamycin discussion, a  product may or may not contain rapamycin and even because it seems to contain rapamycin because it "works" or because of blood levels, how do we know that it doesn't contain toxins because of some faulty Chinese manufacturing process?

JGC in your opinion do you think a couple of synolytic doses would benefit my father in law? Also, Are there medications he is taking that could interfere our interact? 

JOHN 

I think a couple of grams of Fisetin in olive oil taken a couple of times would be good for him.  I'm not aware of med conflicts with Fisetin.

David Hanson 

I just ordered their liposome + cyclodextrin enhanced Resveratrol and Curcumin products from Nature's Essentials.  I'll report any observations when I've received it and  taken some.

On the question of testing, a while back I bought some Lucidias-brand Dasatinib from BonHoa Pharmacy in New Deli, India (at a greatly reduced price compared to Bristol-Meyers-Squibb's $1k per dose).  I provided one tablet from the bottle, and Johnny Adams arranged to have it analyzed high-resolution with liquid chromatography by Echelon Biosciences, as funded (~$200) by his Gerontology Research Group.

The analysis produced a concentration vs. molecular weight plot that was essentially empty except for a huge peak at just at the molecular weight of dasatinib (488 amu).  There was also a tiny peak (<0.05%) at a slightly higher molecular weight, probably caused by a trace of oxidized or methylated dasatinib.  In other words, the Lucidias product was the real thing.

I think if we could put together an order for ~100 grams of rapamycin from a selected Alibaba source, Johnny would probably be willing to have a similar analysis done for a rapamycin sample.  Anyone interested?

JGC I am interested. Thanks for organizing it.

Folllowup on Nature's Essentials use of Cyclodextrins

My order of Nature's Essentials Tumeric/Curcumin just arrived. They are long-ish orange tablets, about an inch long and 1/3 inch wide.  The fine print on the back label of the bottle indicates that the product contains hydroxypropyl-ß-cyclodextrin.   This is a more water-soluble derivative of the ß-cyclodextrin discussed above.

JGC yes

JGC I would be interested in the rapamycin purchase also.  Seattle local.

Dennis 

After making an inquiry about a bulk purchase of Rapamycin on Ali Baba and getting the run-around from several potential supppliers, I decided that ordering it this way was too much of a crap shoot.  I had contacted The Antiaging Store about reports that their RapaPro brand of Rapamycin did not show up in bloodwork after taking it.  They sent me a multipage report from a testing laboratory showing assay results of their product that I found to be fairly convincing.  On that basis, I ordered two bottles of RapaPro, which my wife and I are now taking once a week.  Here is the order LINK.

JGC 

Sorry, wrong link.  Here is the correct LINK.

JGC Why not have your batch of Rapa tested yourself?  If AAS was previously exposed for errors or misrepresentation of their product, why would you trust an analysis they provide?  Documentation is easily falsified.

Dan Nave I am recovering from the flu or whatever it is going around. Still have reduced symptoms. And yesterday I tried one round of Fisetin along with Quercetin  and Senolytic Activator..... I was going to do it again today and tomorrow until I read your post today. I assume your advise would be to wait til all the symptoms are gone with Flu? I did this a few weeks ago and loved it..felt very energetic... today I feel lethargic. Maybe you are correct...

Dorian Gray 

The docs (LINK) look authentic, so I don't think it's worth $100 or so to do the redundant test.

JGC bonhoa.com doesn’t exist. Is this the same people?

https://www.bonhoapharmacy.com/tmsearch?orderby=position&orderway=desc&search_categories=2&search_query=Lucidas&tm_submit_search=

Chuck Frasher 

It does seem to be the same New Delhi pharmacy.  Their prices seem to have gone up.

JGC  Thanks so much. Your reply leaves much confidence. 😊