How to increase the bioavailability of Fisetin
I'm preparing to do a Fisetin senolytic experiment, but I've read on the article from Mayo Clinic ( https://linkinghub.elsevier.com/retrieve/pii/S2352396418303736 ) that the administration mode was done through a mix of phosal and other stuff :
100 mg/kg of fisetin in 60% Phosal 50 PG:30% PEG400:10% ethanol
So the bioavailability of the Fisetin should be a key step for the treatment. I am looking for some information on how to increase the Fisetin bioavailability. Does anyone know of some methods to do this ?
It is claimed that taking 10 mg of BioPerine, a supplement that is reputed to magnify the effects and potency of flavenoids and other supplements, along with a dose of Fisetin will greatly increase its bio-availability. In our last session of taking a massive dose (5 g) of Fisetin, my wife and I took 10 mg of BioPerine with each of ten 500 mg doses of Fisetin. This did seem to produce some magnifying effect, because I experienced a mild side effect (vertigo) that I had not experienced with a previous large Fisetin dose.
I refer you to https://www.isotrope.com/bioperine/ . It says that: "P-glycoprotein is a protein the body uses to break down exogenous compounds found in the body. This protein inhibits the action of many medications, and also regulates the degree to which certain nutrients are absorbed by the body. This protein actively controls the permeability of the blood-brain barrier, which directly impacts the overall effects seen by many compounds such as curcumin—the active compound found in Turmeric. Piperine inhibits the action of this protein."
It isn't clear to me why you think that suppressing the production of the drug breakdown-compound P-glycoprotein should be related to the permeability of the blood-brain barrier.
For what it's worth, except for a mild headache that lasted a few hours my wife and I experienced no subjective brain-related effects after taking 10 mg of BioPerine one hour before taking several large doses of Fisetin or of D+Q.
In my opinion, clearing the senescent cells in the brain and central nervous system is a beneficial thing, and it is good that the Fisetin molecules are small enough to reach the brain. In fact, it is a concern for me that the very promising new general technique for clearing senescent cells that is being developed by Oisin Biotechnologies, which involves using p16-targeted designer-DNA plasmids delivered to cells by special lipisomes, has problems in penetrating the blood-brain barrier because the lipisomes are so large.
JPA, check out the Bulletproof Radio podcast where Dave Asprey interviews Joe Mercola -- episode 588. They discuss creating a customized suppository to supplement with things fisetin and NR specifically, which are apparently absorbed 10x better that way than via digestion.
I'm NOT doing this because it's gross, but apparently it works.
I'm on day 4 of a 5 day x 1200mg fisetin regimen (planned for every 2 months per my doc), and both yesterday and today added 100mg 7,8 Benzoflavone, 100mg resveratrol + 10mg black pepper + 240mg r-lipoic acid + 290mg PS to the 1200mg fisetin. Detox reaction / headache & vertigo for the last 3 hours, although yesterday wasn't as much of a headache. Also consider the deep tissue massage received shortly after today's dose. Also on 6mg rapamycin / week + 500mg Metformin ER x 2.
Je me présente brièvement j'ai 31 ans, je prends du Trans-reveratrol depuis le 1er janvier 2013.
J'ai souhaité faire une cure de FISETIN notament pour les propriétés anti-inflammatoire mais également pour l'élimination des cellules senescentes.
Après de nombreuses lectures d'études et de témoignages j'ai acheté un pot de Fisetin 100Mmg Dr. TheBest et 1 pot de fisetin 100mg. SWANSON. Afin d'être sur d'assimiler la fisetin j'ai pris le produit ventre vide et au cours d'un repas, le matin et le soir et des fois en cours de journée (j'ai tout essayé). J'ai testé la fisetin 500mg au réveil avec Vitamine C + OMega 3 + 30ML d'huile de Noix. Concernant les résultats indéniablement ils existent!!.
J'avais des douleurs au bas du dos et à l'épaule à cause de la musculation elles ont totalement disparues, je constate plus d'énergie et lors de ma cure qui a duré 5 jours ou j'ai pris en moyenne entre 500 et 800mg par jour, j'ai constaté une véritable sensation de "froid" dans mon corps c'est très impressionnant. Je constate une meilleur humeur et un regain d'énergie, plus qu'avec l'utilisation de précurseur de Nad+.
Les cellules sénescentes s'accumulent tous les 60 mois. Je vais donc refaire une cure mais cette fois je vais combiner avec de la bioperine. Je tiens à préciser que j'utilisais la FISETIN toujours en combinaison avec 250mg de Trans-resveratrol. Les 2 premiers jours de la cure j'étais un peu amorphe.
Je vous tiens au courant des effets de la FISETIN + BIOPERINE. N'hésitez pas si vous avec des questions.
I introduce myself briefly I am 31 years old, I take Trans-reveratrol since January 1st, 2013.
I wanted to do a cure of FISETIN notament for the anti-inflammatory properties but also for the elimination of senescent cells.
After many readings of studies and testimonials I bought a pot of Fisetin 100Mmg Dr. TheBest and 1 pot of fisetin 100mg. SWANSON. In order to be sure to assimilate the fisetin I took the product empty stomach and during a meal, morning and evening and sometimes during the day (I tried everything). I tested the fisetin 500mg waking with Vitamin C + OMega 3 + 30ML of walnut oil. Regarding the results undeniably they exist !!
I had lower back pain and shoulder pain because of bodybuilding they totally disappeared, I consume more energy and during my treatment that lasted 5 days or I took on average between 500 and 800mg a day, I found a real sensation of "cold" in my body is very impressive. I consume a better mood and a boost of energy, more than with the precursor use of Nad +.
Senescent cells accumulate every 60 months. So I will do a cure again but this time I will combine with bioperine. I want to clarify that I was using FISETIN always in combination with 250mg of Trans-resveratrol. The first 2 days of the cure I was a bit amorphous.
I want you to avoid the effects of FISETIN + BIOPERINE.
Do not hesitate if you have questions.
I found this report from 2016. Some are obviously trying to increase bioavailability through nanoencapsulation. I hope someone soon has an efficient route for placing fisetin in the bloodstream.
Some have proposed using suppositories. Suppositories can be ok if the only purpose is to do intermittent senolytic therapy. But for daily use I prefer the oral route. I have not heard about any experiments with homemade fisetin-suppositories. I guess we have to see if anyone is up to it.
One suggestion is to add lecithin to the dose of Fisetin. Lecithin is well known as an emulsifier so it should help mix Fisetin with water and increase absorption. I tried it with my last course of senolytic meds using approximately 2 grams of Lecithin with each 2 gram dose of Fisetin. I also take Echinacea as an immune system stimulant on the assumption that Fisetin exposes senescent cells but it's the macroglia that actually remove those cells. It seem logical that temporarily stimulating the immune system to produce more macroglia and increase their activity level would make better use of the Fisetin while it's effect lasts.
Has anyone seen / heard of a simple test to determine what concentration or percentage of senescent cells are present in a body or some indicator of how much have been removed? Without some metric to measure the level of senescent cells in a living body it's hard to judge how effective any senolytic treatment is.