How to increase the bioavailability of Fisetin
I'm preparing to do a Fisetin senolytic experiment, but I've read on the article from Mayo Clinic ( https://linkinghub.elsevier.com/retrieve/pii/S2352396418303736 ) that the administration mode was done through a mix of phosal and other stuff :
100 mg/kg of fisetin in 60% Phosal 50 PG:30% PEG400:10% ethanol
So the bioavailability of the Fisetin should be a key step for the treatment. I am looking for some information on how to increase the Fisetin bioavailability. Does anyone know of some methods to do this ?
It is claimed that taking 10 mg of BioPerine, a supplement that is reputed to magnify the effects and potency of flavenoids and other supplements, along with a dose of Fisetin will greatly increase its bio-availability. In our last session of taking a massive dose (5 g) of Fisetin, my wife and I took 10 mg of BioPerine with each of ten 500 mg doses of Fisetin. This did seem to produce some magnifying effect, because I experienced a mild side effect (vertigo) that I had not experienced with a previous large Fisetin dose.Reply
I refer you to https://www.isotrope.com/bioperine/ . It says that: "P-glycoprotein is a protein the body uses to break down exogenous compounds found in the body. This protein inhibits the action of many medications, and also regulates the degree to which certain nutrients are absorbed by the body. This protein actively controls the permeability of the blood-brain barrier, which directly impacts the overall effects seen by many compounds such as curcumin—the active compound found in Turmeric. Piperine inhibits the action of this protein."Reply
It isn't clear to me why you think that suppressing the production of the drug breakdown-compound P-glycoprotein should be related to the permeability of the blood-brain barrier.
For what it's worth, except for a mild headache that lasted a few hours my wife and I experienced no subjective brain-related effects after taking 10 mg of BioPerine one hour before taking several large doses of Fisetin or of D+Q.
In my opinion, clearing the senescent cells in the brain and central nervous system is a beneficial thing, and it is good that the Fisetin molecules are small enough to reach the brain. In fact, it is a concern for me that the very promising new general technique for clearing senescent cells that is being developed by Oisin Biotechnologies, which involves using p16-targeted designer-DNA plasmids delivered to cells by special lipisomes, has problems in penetrating the blood-brain barrier because the lipisomes are so large.Reply
JPA, check out the Bulletproof Radio podcast where Dave Asprey interviews Joe Mercola -- episode 588. They discuss creating a customized suppository to supplement with things fisetin and NR specifically, which are apparently absorbed 10x better that way than via digestion.
I'm NOT doing this because it's gross, but apparently it works.Reply