Engadin Zermatt Thanks for the site. My supplier as well. I did reorder a couple of weeks back via Alibaba, but that might not be possible moving forward.

Engadin Zermatt Engadin Zermatt

I wonder if you have a special contact person at Wuhan Hengheda Pharm Co? Or do you use the company email? 

Staffan Olsson Yes, Sanny Zhang. And I have this message from them since April this year:

"Hi, this is Sanny from Wuhan HHD Pharm, manufacturer of nootropics, anti hairloss, sports nutrition and herbal extracts.

Our shop might be closed by Alibaba since May,2021, our business will be transferred to other web, in case you have any products interested that would like to inquiry from us in the future, pls contact email/skype: hhdpharm05@outlook.com, thank you.

whatsapp: +86-13129973353"

If you want to mention me as a reference I am Gonzalo Yebenes.

I guess the new web she mention is made-in-china.com.

Steve Roedde 

At the moment I don’t use rapamycin but will try o buy rapamycin powder from China and I would be grateful to hear how other people make sure they ingest the right amount powder.

I would like to avoid dealing with the powder in mg doses. This even if I have the option of using a mg scale.  I would like to find a better way to take the right amount without dealing with a scale every single time.  

I see that rapamycin is sold in ready made solutions with DMSO. That makes me think that one way of administering rapamycin powder bought from China is to make your own DMSO solution. Any ideas about this and if it is a useful approach. I am concerned about the stability in DMSO oand how long it can be stored in DMSO?

https://www.sigmaaldrich.com/SE/en/product/sigma/r8781

Staffan Olsson It’s pretty simple. You mix 1 gram of Rapamycin with one kilo of lactose (I posted instructions somewhere on this site). You do indeed need a mg scale to weigh the Rapamycin.. to make sure it’s exactly one gram... a kitchen scale probably close enough for the lactose. This will give you 1 mg of Rapamycin in one GRAM of lactose. Then weigh the weekly dose you want to take. Measure the volume (5 mg likely ~ a tsp). Then just measure the volume. If you get 5.5 mg one weeknand 4.5 mg the next, it probably doesn’t matter! That’s the way I do it. 

Staffan Olsson Steve Roedde I used chat feature on Alibaba. Whoever they have answer the main chat immediately routed my query another person that negotiated my order, after I stated what I was looking to source.

As for mixing it down, I've been surprised no one has gone with solutions. I'm concerned about getting a homogeneous mixture putting 1 gram of fine powder into 1 kilogram of another fine powder. Rapa is water insoluable, but it dissolves in both DSMO and ethanol. With a solution, there's no mess and hassle filling capsule and finely adjusting one's dose is trivial too.

My plan is to mix 1.5 g into 750mL Everclear. That will give me 2mg/mL or 10mg/tsp. It hadn't occurred to me before now that the rapamycin molecule might decompose in solution, but now that I think about it, I suppose that's a possibility. If anyone knows if that is true, please let me know.

Again, I'm surprised solutions are not the most common approach. Is there a reason besides pills are what doctors, pharmacists, and customers are most used to dealing with... if you're dealing with the general public pills are preferable because people are less likely to mess up the measurement.

Steve Roedde Probably a silly question, but what if you are lactose-intolerant?

Also, to clarify, you stated that you mix 1 gram of rapamycin with 1 *kilogram* (2.2lbs) of lactose?

Keith Abe you can use another filler (dextrose for example),  if lactose is really a problem, although 5 grams probably won’t be an issue. You could mix it at 2:1, 1000 mg (1 gram) of rapamycin, in 500 grams (1/2 kg) of lactose (or dextrose), giving you 2 mg Rapamycin per 1 gram of lactose. 

The final dose at 5 mg per week would then be around a half tsp. 

As it turns out, I had to mix another batch, and only had dextrose around. It worked fine, but is awfully sweet (the rapa is pretty tasteless). I made it 2:1 to refuse the glucose load. 

Steve Roedde Thanks for the reply, Steve. I admit that I am having a difficult time imagining how I can add 1 gram of rapamycin (or any powder) with 500 grams of another powder, and have the smaller amount of substance x integrate uniformly with the greater amount of substance y. Is there a secret to mixing the two together?

Keith Abe I posted that elsewhere on the forum. Briefly. You get a stainless steel bowl, mix by hand, for 10 minutes. 1 gram of Rapamycin with 10 grams of lactose. Then you add, say, 50 grams of lactose, another ten minutes, then 100 grams of lactose, then the rest of the lactose. The early dilutions are most critical to ensure even mixing. You can put the whole lot in two ziplocked bags at the end, and do more mixing while watching a video or something. Make sure the humidity is low.

Steve Roedde Thank you!

Steve Roedde I have had experience mixing rapa and lactose powders with good results.  Here is a video showing the correct way to do it. 

https://www.youtube.com/watch?v=k-3DoyKy0zE They are using a mortar and pestle, but a SS spoon and large bowl work very well. It takes time (2.5 hours for me to mix 1.5 grams rapa with 500 grams of lactose) You are basically doubling the mixture in bowl in each cycle mixing well between cycles. I now have a 2-3 yr supply. I store my rapa mixture in a glass container in the freezer and take out appx. 3 months supply in a small container for the fridge. 500 grams of lactose was really too much, 250 would have been enough in order to weigh accurately. I weigh mixture with a jewelers scale (1/1000 accuracy) from Amazon that cost around $30. Plenty accurate for this. 500,000 mg/1500 mg = 333.333 mg of mixture to equal 1 mg rapa. So 5 mg rapa dose = 5 x 333.333 = 1,665 mg. or 1.65 grams of mixture to weigh on scale. Weigh in a small plastic container, tip it up into mouth and wash down with a little water. No problem.

Van useful. Similar method. Because I found weighing it every day a POA, having a larger proportion of lactose (or whatever), convenient because the volume is easier to measure with a spoon, I did it that way.

When I started I was taking the rapa daily (it’s for treatment not longevity). I’d weigh the daily dose3-4 times, see what the volume was and consume that. My trough levels were always the same confirming adequate mixing as well as accurate enough dosing. 

With weekly dosing, weighing would not be an inconvenience.

The jewellers scale is of course critical for the initial rapa weight. I had ordered 1 gram ... but was sent over 3.4 grams of rapa. 

I was more descriptive of the with my other post on compounding ... but it could be anywhere on this site. I thought it was on this thread, but apparently not. 

I just knock back the dry powder too. One great mind (you) and I... think alike😉. 

Make a great day. 

Steve

Steve Roedde I also might mention, I use the grapefruit juice protocol to take rapa mixture, and follow the dosing schedule of Dr. Blagosklonny and Green.  They are taking 20 mg rapa every 12-14 days to get "spike" of rapa into the brain to get max longevity benefits.  The high dose allows the rapa to get past the blood brain barrier into the brain where aging is regulated.  I do get sores on mouth/tongue when I increase my rapa dose to 8 mg., but anything below that zero side-effects.  Been on rapa for 4 years.  This GFJ protocol increases dosage to 300-350%.  I purposely wanted to check if I had actually received rapa from China or not.  There is Sirolimus in my mixture for sure.  If you think about the high dose, it is not much different than taking 7-10 mg weekly which many do.

Steve Roedde Inositol works as well. Many years ago when I had a health food store various people would purchase it to cut various illegal substances with

Steve Roedde I see it's listed for sale in a solution with DMSO. So, probably doesn't degrade immediately.

https://www.sigmaaldrich.com/US/en/product/sigma/r8781

In any case, moot for me at least for the next year since I mixed 1 gram into some potato starch I had handy. I made it so 1/4 tsp is 10 mg rapamycin. I plan to take that weekly to start and may adjust if I feel the need.

I have one lingering issue with my knee and elbow joints I'd like to see resolve. If that happens I'd be quite pleased.

Liam_Cohen I see this "Unless otherwise indicated, solutions are probably best prepared fresh and protected from light."

R0395pis.DOC (sigmaaldrich.com)

It would suit me to have rapamycin in DMSO solution. I will look for further information about storage and degrading.  

Mark Rittman What is a rapamycin online prescription?