Rapamycin Cost
Let me clear some things up for people considering rapamycin (generic sirolimus). I am taking it and so are my dogs!! (I have a very open-minded vet who trusts my judgement.)
Dr. Green, a very impressive individual, gave me my personal prescription for sirolimus. I have a very good PPO (Blue Cross/Blue Shield). I took it to CVS, the affiliated pharmacy, and got a great price of about $2.90/mg. (Remember, you are only going to take about 3-6 mg/week.) With a PPO you can probably do this also. CVS wants to meter it to you with a month's worth of doses at a time, but that's just how they do things. You still have access to the full quantity prescribed.
With the prescription from my vet for my dogs, I was forced to take to the open sea. Armed with only a prescription and no insurance, the picture is quite different, but you can still get a good price. Cutting to the chase, I ended up at Walmart. The prescription was for 90 mg - price $1440. But wait! after presenting a coupon downloaded from GoodRX (that's right, just anonymously downloaded and printed) - price $396!!!! As my vet remarked, that's a hell of a charge for not looking around first! ($4.40/mg is better than taking a risk buying online, in my opinion.) Costco is pretty close to that price. At CVS/RiteAid, etc., you will pay vastly more.
You can't touch the original drug rapamune (Pfizer) unless you are wealthy or go to Canada. The problem with the online pharmacies is that a lot of them are scams. Just because they have a website doesn't mean anything. At least check to see if they have a brick and mortar location. Example: At one point I was excited by the online infomercials and wanted to take NMN instead of NR; however, when I looked up the reputations of the suppliers only one was unsullied, and on further investigation their brick and mortar location turned out to be a residential dwelling!
FYI, more than one company makes sirolimus. From CVS the sirolimus was made by Greenstone, LLC, apparently a wholly owned subsidiary of Pfizer.
Hope that helps some people who are considering rapamycin but think it might be out of their reach. If you know a doctor, great, but if you don't a visit to Dr. Green is worth the trip, and I live in CA! (round trip less than $300) Do your homework first and you will learn a great deal.
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I'm going to get another 1g sample from the Alibaba supplier and give the skin lotion a try... I don't think accurate measurement is all that important, Blagosklonny implies that Rap isn't toxic and most likely can't overdose on it as he references a case where a woman tried to unsuccessfully commit suicide by downing 100 1mg tablets with no effect. I think I'm just going to get a micro spoon and try to get a "pinch" in it Lol and just mix it up in the palm of my hand with my favorite face conditioning lotion and apply. Just not sure how frequent to apply it, I'll read the article and see what the protocol was for the study.
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Peregrinatum Your assumption that you cant overdose topically is wrong. Read the study and you will be very afraid of what happened with higher doses
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I just received my second order (same supplier as my first) ...10.48 grams... for ~$650 Canadian. At the price I was paying for "Rapamune" brand in the local pharmacy... over $100,000.00 Canadian. I had been a bit concerned about supply chains... anyway, it's happy in my freezer. As before, I'll compound it, take it (back to my 1mg per day dose) test my levels, and if they are the same as all of my initial tests at that dose, I'll assume it is the real deal. Of course they supplied a COA... but I no longer trust any supplier... but my confidence in the one I have used is growing.
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Steve Roedde yes Steve, same with mine a COA. I asked for HPLC and they said they will get me something. I used to work at a huge Chem company, typically in China, they use high quality inputs from the EU and perform the final batch cooking, so it should be pretty high quality and I am expecting some stainless steel trace element from the vessel. Any extra should be unreacted inputs. I know in India they prefer to synthesize their inputs too and tend to have poorer quality...at least thats how it was 15 years ago when I left..(also explains the huge made in China Pharma). I may have access to a lab, but we do not have pharma fingerprints for comparison so will just have to go on the basic chem inputs and look for contaminants, heavy metals, etc. But not expecting to find any.
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Just received my 10 grams, awaiting some #5 capsules and capsule loading apparatus.
Skin cream: I looked up Dr. Green's site and he has been formulating his for his solar keratosis with 15mg/oz in Aquaphor cream. So I wanted something a little weaker and easier to work with so I used 10mg/oz, each tiny amount on my smallest stainless spoon is 3mg (microscopic amount), so I weighed out 40mg and melted it in DMSO 4-5 drops. Then I poured this into 4 ozs of ArboTrex (70/30 DMSO/Aloe). Saw it in other places on this site. Used red pill mix, which melted in the DMSO to provide a mixing quality agent and I have a wonderful pink skin cream with a pretty high concentration and it actually is a solvent that penetrates skin pretty quick. I have solar keratosis in a few spots on my hand and forehead, should see something in a few months.
I am waiting on taking Rapa pills to get a baseline test to see if there is any absorption into the bloodstream from the DMSO. I would expect some if the molecule is small enough...
On over indulging, after seeing how small a few milligrams is and how potent this is I want to be careful. A safe weight loss regimen is up to 25mg one time dose in 2 weeks, so even if we do get absorption, it would take the entire 4 oz of cream to get interesting....but, if I double up with absorption and low dose pills it may be too prolonged in my system and hit MTOR2, which I would like to avoid.
I'll let you know my results as the data comes in.
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Steve Olsen where can I learn more about the safe weight loss regimen? 24 mg???
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Julie Anderson google
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Julie Anderson hi Julie, this site and a couple others and I think Bloggoskiny have mentioned high dose for weight reset. My wife tried 25mg and she lost a few lbs that month but no more. At 5 to 6mg I have lost 10 lbs in three months. My wife also did a 4 day fast, which was a small improvement but not lasting so I think she needs a higher dose or longer fast for a better hormone reset. One other point, she is near menopause and it seems to be regular again so maybe this is getting in the way? My 88 mother called after two months and was clear for the first time in a couple of years! My solar keratosis on one hand have disappeared after 4 months and face is Improved after what appeared to be degradation which I think is a transition similar to retin A, first it kills bad cells then improves and replaces them. I will have my mom run Hematocrit and start on ip6 to see what's going on there based on this log. She has been on 3mgs per week and I want to up it to 4. My dog is next. Good luck to everyone.
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The home handyman's guide to using pure Rapamycin powder.
1. Weigh .1g Rapamycin.
2. Cut ends from 20 Splenda packets. Save packets. Empty contents into small round bottom glass bowl. (Dessert dish). Yields approx 20 G.
3. Add Rapamycin to Splenda bowl. Mix 3 minutes with kitchen electric mixer paddle chucked into a variable speed cordless drill. Use low speed.
4 .Weigh resulting contents. Divide by 20. Weigh out that amount 20 times placing each portion in one saved packet and seal each. Mix again after each 2 or 3 portions.
5. Yields 20 - .005 G doses to be used in Sunday morning coffee for the next 20 weeks.
Maybe a bit Redneck but it gets the job done. Your mileage may vary.
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Just ordered 30@3mg compounded Sirolimus from Tailor Made Compounding with a script from Dr Green. Cost, including shipping, was USD $97.50
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I've been reading these postings, some of them old, some of them recent, with interest. I must say that that there are different concepts of what slowing down aging means, and people are arguing about methods, when in fact they are really arguing about the meaning. Someone believes that slowing down aging means preventing the physical decline of muscle (sarcopenia) by taking HGH. Someone else believes in slowing down aging at the molecular level by mimicking calorie reduction through Rapamycin , or by actually fasting. I've always felt that if slowing down aging meant that I had to weight 140 pounds and not be able to pick up my grandchild off the floor, I probably did not want that for myself. It does seem from all the research articles I've read that in order to accept a more youthful molecular makeup, you have to sacrifice the actual advantages of youth (e.g. strength, speed, food). I don't think that either extreme is useful. In my case, I am 64 years old, but my biological age is 10-15 years younger. I attribute it to my being a weightlifter since I was 18, and not smoking, drinking excessively, or doing any drugs. When much younger and thinking of becoming a professional bodybuilder I turned down steroids, and HGH, because I did not believe they were safe. And from the experience of friends over the years, I was probably correct in that assessment. At 64, I have no high BP, no evidence of cardiovascular disease, no diabetes, no prostate problems, no ED, and only recently developed gray hairs. I can do things at my age that people half my age wish they could do. But 2 years ago, when I saw my HA1C creep up to 5.5, I started taking Metformin preventively, and I also started doing a modified 16-8 eating window. So I'm not averse to trying things. My point is that there are tradeoffs, and the goal should be to live longer with a reasonably youthful molecular makeup, while not having to be a weak, puny 140 lbs. If that means sacrificing some years, I would be for that (but I realize not everyone may share my sentiment).
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roger larre I am currently 74 yo., taking Rapamycin for 4 years. I lived a similar life as you did when I was younger. Was running, 3-4 days a week, 5 miles runs at 64. Then I turned 65 and my stamina and recovery time fell off a cliff. Hope you can delay it longer than I could. My calculated biological age is 47. I feel good, but still do not have all the energy I would like.
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Van Take a complete blood count. It might be that your red blood cells have dropped, and that is why you are feeling weaker. check for "hematocrit" and
"hemoglobin." see my page: http://www.rajeun.net/hb.html
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Ellis Toussier Ellis Toussier Hematocrit 41.50% Hemoglobin 14,000
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Hello Van... well, there it is... "Average" Hematocrit is 45%, and yours is only 41.5%... although almost all "doctors" would tell you that's "okay", I am not a doctor but I have 20 years experience with feedback from people that have less than 45% Hematocrit, and they all feel the same... BY DEFINITION, it is still not called "anemia"... but you are very close to the definition of "anemia"... Look up "anemia" on Google, and you will see that anemia is associated with many other degenerative conditions.
To begin with, all of the NEURONS in your body need OXYGEN... And less oxygen, means some of those neurons suffer. You were born with all of your nervous system... but for various reasons, some of our neurons die... we lose them...
But you should avoid at all costs to lose neurons, because WE ARE our nervous system. As we lose neurons, we become slower, we lose memory, we lose abilities.
That particular lost neuron will never be alive again. And as we get older, we lose neurons for many reasons... perhaps by accidents, perhaps by poisons, perhaps because the circulatory system gets blocked up by sugar and so blood (and oxygen) does not reach certain neurons... goodbye, neurons. Neurons can be substituted
by other neurons, but every neuron has a "memory" and once that memory is lost,
another neuron might try to replace it, but it is never quite as good as before.
That is "normal Aging"...
But you don't want to be "normal". You want to be "abnormal"
you want to NOT lose neurons. And with low red blood cells, you WILL lose neurons.... slowly... but you have to try to not let it happen. Go see a
hematologist, ask him to help you to raise your red blood cells.
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Ellis Toussier I believe I will do that. Will not tell him I have been on Rapamycin for 4 years. I eat a lot of red meat, I am keto, 71", 165 lbs. which is perfect for me. So getting iron and also take B complex mainly for folic acid and B2 which are needed in red blood cell development. Will report back
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I have seen numerous cases of low hemoglobin in the elderly, including my grandmother and my father, and also elderly friends. The medical community seems to believe that because it's in the elderly, it's ok. In 1998, my grandmother, 89 yrs old at the time, was hospitalized with low hemoglobin and some dizziness. She was given a transfusion which bumped up her hemoglobin and made her dizziness better, but it made he breathing more difficult. As the days progressed and her hemoglobin dropped, her breathing would get better but her dizziness would return. She was put through all kinds of test to eliminate the usual causes of low hemoglobin but they could find nothing. She was transferred from one hospital to another. Meanwhile, they continued to give her transfusions every 3-4 days, with the same effects as explained before. Finally one day she became septic, probably due to her immune system being compromised because of the regular transfusions, few days later developed general organ failure and died. At our insistence, they did an autopsy and discovered two small lesions in her heart, each about 6 months old (which made me recollect an episode of radicular arm pain she had had for a while). In other words, she'd had 2 small heart attacks, which had probably left her with congestive heart failure, which most likely was causing a bone marrow insufficiency leading to low rbc/hemoglobin. My father has also been living with low hemoglobin for 3 years (12.7 g/dL), and I can't seem to be able to convince anyone to treat it with Erythropoietin (I realize it's expensive). "Coincidently", during the last 3 years he developed MCI and now AD. I do wonder if anemia is incidental to AD. It can certainly make matters worse.
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roger larre A major cause of anemia in seniors is low Testosterone levels. I do not know if your relatives had their T tested but this is one of the first things a good gerontologist will do. Second, low T and estrogen are major contributors to dementia. You can verify contribution of steroid hormones to both of these issues with a short Google inquiry.
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roger larre Sorry to hear about your grandmother and father.
Recent review article on the issue:
The Impact of Low Hemoglobin Levels on Cognitive Brain Functions
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723430/pdf/cureus-0012-00000011378.pdf
Many association theories, but no direct causation proof.
Actually, indirectly, I am in the "dumping iron" camp...trying to reduce my iron stores as low as possible without causing anemia related conditions. Iron is a pro oxidant, and associated with cognitive diseases (Alzheimer, Parkinsons). It's a simple and grand theory that might also explain why women live longer than men (men have continuous buildup of iron stores, whereas for women, only after menopause, which is when their risk of CVD increases).
https://roguehealthandfitness.com/category/iron/ (a good review of iron implicated in many diseases)
Haem metabolism is implicated as pro aging.
Multivariate genomic scan implicates novel loci and haem metabolism in human ageing
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366647/pdf/41467_2020_Article_17312.pdf
I freely donate 500mL of blood every 8 weeks (the maximum frequency here), and my Ferritin is in the 15 level. Males have no reason to have a Ferritin higher than 50. Most people are walking around with Ferritin FAR higher. My doc is "freaking out" that my Ferritin is so low, but he's old school trained. I am teaching him many new things related to keto & thyroid, iron, fasting, aging. I am a heavy daily exerciser in excellent cardio/muscular shape. I eat animal protein, nuts, dark chocolate so I have no worries about depleting my iron levels.
Donating blood is also a way to dilute "old" blood and rejuvenate, which is implicated in some exciting new work on blood dilution/young blood aging factors as pro longevity (extensions of Paribiosis work on old/young mice pairings)
https://www.lifespan.io/news/conboy-interview/
http://www.programmed-aging.org/theory-3/Katcher.html
https://www.longevity.technology/new-young-blood-plasma-research-creates-a-stir/
My thinking is that if you can go into old age in SUPERB physical shape, watching biomarkers, then excess iron is NOT a requirement, and in fact, may be pro aging.
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roger larre most doctors don't understand anemia. Even here, on the age-reversal forum, a "doctor" said that hemoglobin 13 in an Old Man (Dr. Green) is okay because it is "equivalent to 14" in a younger man... and he said that low hemoglobin is "normal" in old men, so therefore it is acceptable. Completely, totally, he and most doctors don't understand anemia, or the harm that it does. It doesn't HURT (and it doesn't... ) so they don't try to fix it.
And they are AFRAID of EPO. Somewhere they read that EPO "thickens the blood" and they are scared that they will cause a heart attack. Yes, EPO thickens the blood... but too thin blood is also bad. They don't think about that. Your grandmother would have been much better off if they had injected her with EPO, than with blood transfusions.
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roger larre Blood cells are made from hematopoietic cells, we are born with about 20,000 and as you age they die or become senescent. A woman that died when she was 115 years old had only 2 hematopoietic cells left to make her blood cells. So this seems likely the cause of low hemoglobin in the elderly.
This study looked into it and found it wasnt testosterone and said it was aging or unknown, seems strange, sounds like lack of functioning hematopoietic cells.
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Fred Cloud Fred, there are multiple studies that document testosterone treatment alleviates anemia in senior men. Just google the topic and see what happens. This is standard protocol for gerontologists that I was introduced to 20 years ago.
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peter_h_h@owe
Thank you. But it's very difficult to get a doctor to think outside the conventional box. Treatment of anemia associated with CKD (renal insufficiency) is acceptable. But his renal functions are pretty good. I'm actually trying, but so far have failed to convince my father's GP to use erythropoeitin to treat his anemia. I also asked a geriatrics doctor, with the same results. His anemia is not considered serious enough, and the insurance company probably will not pay for the expensive treatment. But I will look over his medical history to see if T was tested. If it's low, I will probably run against the same resistance.
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roger larre Roger, if he does not have the blood test, you can get a male basic panel from Life Extension for $75 and a CBC/Chemistry panel ( includes iron, but not ferritin)for $35, if you are close to a Lab Corp. I do not know where you live, but there are multiple Drs. who will prescribe compounded testosterone from a local pharmacy and it is not very expensive. I would look up compounding pharmacies on the internet, visit the pharmacy and get the names of Drs. that use the pharmacy. It does require a script and insurance will not pay for it. Note that compounded T is not the best. If you have a copy of Life Extensions" Disease Prevention and Treatment" there is a chapter on ideal male hormone levels. You can purchase DHEA-S and pregnenolone from LEF for a few pennies/day. The male panel includes DHEA which I believe is as important as T in many ways. It also includes estradiol which may be even more important than T for senior males. Males get estradiol from aromatization of our T in the liver.
I know that medicare will not pay for a lot of this, but there are ways to keep the cost down. It is also something for you to consider as you start to merge with the setting sun.
I do not agree with MAC in that it is my opinion that maintaining optimal hormone levels is critical if not essential to preventing dementia, sarcopenia, osteoporosis etc. in seniors and there are multiple papers on this. There are certainly other actions one must take which include vitamins, fish oil, exercise and proper diet ( no simple carbs and at least 12 hours of fasting).The motivation to do this , in my opinion, depends on ones hormone levels.
Finally you may want to consider fisetin and follow Mayo's protocol. I am 80 and found this supplement to be "miraculous". Mayo is using this in 3, phase 2 trials to prevent frailty in seniors. It is cheap and I buy from Swansons. There is a thread in this forum where a number of individual feel the same way I do. However, like all issues, there are some of who disagree.
Attached are just some of the multiple papers on these topics.
https://academic.oup.com/endo/article/151/2/628/2456433
https://www.agelessmenshealth.com/low-testosterone-levels-predicts-dementia-risk/
http://www.encognitive.com/node/12840
I apologize for the wordiness. Suggest the first step is the blood test and go from there.
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Peter H. Howe I was referenced in your post about "not agreeing with MAC"? What exactly do you disagree with? Was it with regard to iron levels? I think you referenced hormones, but I haven't posted anything about hormones? But for the record, I am under the care of an FMD who specializes in hormones for pro-aging, and have been on DHEA and injection Testosterone for years (55 yo male). I (my doc) monitors ALL my hormones like a hawk for OPTIMAL health.
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MAC Mac, your post implied that donating blood could be a remedy for Roger's fathers anemia. This is unlikely to happen and I note that Red cross will not accept blood from an anemic person. I also note the first paper you posted indicated that anemia in many studies is commonly associated with cognitive decline in seniors which includes Alzheimers, but as you suggested this may not indicate causation.
Given this association, it would not be advisable to increase anemia. The papers and the LEF book I referenced for Larry to review clearly document that testosterone therapy for anemia and cognitive decline may be a viable option to treat his father. The LEF book also has section on treatment of anemia and recommends among other options testosterone treatment.
My suggestion to Roger was for him to have have his fathers T tested ( along with DHEA and estradiol) and go from there with reference to LEF's book as a basic reference. I also included a suggestion as to how to proceed if his father is resource limited or how to find a physician.
There are multiple reasons given why women on average live 5 years longer than men. One is that women are smaller than men and as a consequence undergo less free radical damage over their lifetimes. . Second is that women in general have less demanding/health compromising physical jobs. Always remember you are comparing all women versus all men.
A better argument is that males that are neutered early in life will on "average" live 20 years longer. This association was speculated to be due to improved immunity as testosterone is a contributing factor to the decline of the thymus- but who knows. There are also numerous option/methods to improve ones immunity, and that is another effort.
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Peter H. Howe I see, you completely misunderstood my post, my bad. Did not imply that in the slightest. I was only discussing completely separate topic of donating blood to dump iron and for rejuvenation/pro longevity.
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MAC Thanks . Forgot to mention in previous post that women who get hysterectomy also live ~5 years longer than other women.
I am looking at plasmaphoresis. I am border line anemic and prefer not to give up my RBC's. Suspect will give it a try if current accolades continue. Keeping up with Conboys etc. Waiting for someone on this forum to report out as occurred with fisetin.
Have a good year.
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I just ordered fisetin looks like there are a couple of protocols, 1 gram per day for a few days or 500mg per day for 5 days. I'm going to try the 5 day one first.
Have made the rapa skin cream with arbotrex dmso product at 10mg per oz of cream. Works great on hands and arms, but too harsh for my face so I mixed up the same with a derma e hydrating face product which seems better. Only 2 weeks in on this and sore spots that were always festering have started to heal for the first time in years. Keratosis spots seem to be softening.. taking pics every week to document.
Took some rapa the last two weeks started low 3mg. Will continue for a month or two and increase probably to 5mg per week. Wife got some extra energy, me no difference yet.
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Thank you all for the various suggestions. Let me mention that my father has been tested for all possible (known) causes of his very mild anemia, and all proved negative. No Iron deficiency, no B12/Folate deficiency, no malignancies,..etc. It does not seem wise to me to further burden his frail system by having him donate blood in order dilute his plasma. It would worsen his anemia, and the body will not be able to reconstitute his blood quickly enough. In a younger person it might make sense though. His GP has told me to take him to a Hematologist or Kidney specialist if I want him to be treated with Erythropoeitin. About Fisetin...I've been taking 200mg daily for 8 years as a preventive. Since late onset Alzheimer's seems to run in my father's family, I am concerned about it so I myself take it, and I've been giving it to him for about 3 years. R Alpha Lipoic Acid (600mg daily) and D3 (5000 IU or enough to boost levels above 50,000) are also part of our staple. In addition, I've had very good results recommending Benfotiamine to my father and friends who suffered from T2DM peripheral neuropathy. Their neuropathy completely resolved after several months of administration. In my opinion it works better than the other recommended regimen, B12/Folate. One particular friend, who's had T2DM for 30 years, was on his last legs (no pun intended) with foot pain and numbness caused by neuropathy. 6 months of Benfotiamine administration completely resolved it. And now I see it is in clinical trials for Alzheimer's also. I will say one other thing concerning Alzheimer's, if anyone is concerned about it. I was suffering from slight memory issues a few years ago, and also issues with concentration. I discovered a brain game called Double Decision, from Posit Science, which exercises memory and peripheral vision, and it did wonders for me. I've been using it for 4 years. It is the only cognitive training proven to reduce the incidence of Alzheimer's by so much (50%). NO ther brain game or cognitive training has proven it can do this. BTW, I am not connected in any way or benefit financially from recommending this, in case anyone wonders. I am including a link to the research in case anyone is interested.
https://www.statnews.com/2016/07/24/brain-training-cuts-dementia-risk
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roger larre Great insights, thank you. How much was the daily amount of Benfotiamine needed to help with the foot pain caused by T2DM? Do you remember? I have a friend suffering from this. And...if you have any other vitamin/mineral supplements you'd recommend in concert with the Benfotiamine, to help with the foot T2DM, I'd be grateful:)
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Kenneth James Brady From all the research I have done, neuropathy relief occurs at a threshold dose of somewhere between 600-900mg daily. You might have an effect at lower doses, but if you dont then dont give up until you try doses in that range.
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Fred Cloud Thank you!!
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Perhaps nicotine chewing gum can also help to stimulate neurons, to "remember". But nicotine can also be habit forming, so be careful not to overdose. start with 1/2 gram, two times per day.
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Update:
The aggressive Rapa cream is working great on my Hands, Arms and the non DMSO product is helping on the face, but not as fast, as expected. Looks like even hard solar keratosis is going to be healed within 2-3 months, which is better than Retin A which I tried for 5 years!
Took the Fisetin, did not get any reactions, but have been on Resveratrol for a couple years, did up to 5 day water fasting, so that probably already took care of those old cells.
Taking the Rapa weekly, feeling OK, some up and down days as usual, but need to increase weight lifting again....to minimize sarcopenia.
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Steve Olsen Hate to ask but do you have any before and after pics to share? TY
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Michael I am tK8ng
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Michael I am taking pics every other week it has been less than 2 months. The dozen keratosis are definitely softening and appear thinning. I will be happy to provude some after they improve as much as possible. I also have pics of my face which is improving slower but festering small sores have healed over Already. Others that were turning into keratosis seem to be healing too. I have age spots which do not seem to be lightening but there is laser lightherapy that should work well for this. Give me another month or so and I will put he photos together with a time series for reference. Then I will make them available with my formulations for reference.
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Steve Olsen BEAUTIFUL! Glad to hear you're having such nice results.
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I wanted to post an update from my original post here:
https://forum.age-reversal.net/t/63jmdc?r=m1hmzzm
I have been taking weekly Rapamycin for 3 months now and I am very satisfied with what it is doing for me and how it makes me feel. In general, my weekly dose of Rap helps me get a much better night of sleep, it evens out the peaks and troughs of energy for me throughout the day (no cat naps needed anymore), more endurance of energy into the evening, better recovery after strenuous work or exercise, increase in mental sharpness/clarity, and an increase in motivation to get things done (daily tasks, projects, etc.)
First task was to determine my ideal dose, so I started with 5mg per week, and increased this 1mg at a time each week, I did this up through 13 mg... I weigh 79 kg so this peak dose is 0.165 mg/kg below the max of 0.2 mg/kg I was willing to push it... each week I would look for the side effects listed in the 2014 Manick paper, I never did get mouth ulcerations but at 9mg and higher I would get a headache a few hours after taking the weekly dose (I rarely get headaches)... headaches and mouth ulceration are the most frequent side effects listed by Manick... Now I have settled on 7 mg per week (0.088 mg/kg) and may go to 8mg, and no side effects are occurring, except for one...
I found that the weekly Rap was increasing my blood pressure, this is a result found in several studies (seems that almost 40% have experienced this)... my usual blood pressure is 125/82, but with the weekly dose it got as high as 150/90, enough of a concern for me to stop taking Rap for a week to see if my BP would return to normal, which it did. Further research seems to indicate that over the first 6 months to a 1 year BP will return to normal for most. I am monitoring my BP a few times per week, and willing to take the next year to see if it returns to my normal levels, the benefits of Rap are worth it as far as I am concerned! When I went off the Rap for a week I could really feel the difference of not having it, if you are taking Rap now take a week off and see how it makes you feel not to have it. I would say it took about 2 months for me to start noticing the benefits of the weekly Rap.
I also made some Rap skin lotion to treat my face and hands (on Dr. Green's website he gives instructions on how to do this)... Face for age spots and wrinkles, hands for sun/age spot. I mixed up a 0.002% and 0.02% mixture and use the 0.002% 4 times a week or so as an after shave, and the 0.02% 2 or 3 times per week for trouble spots... I have been doing this for a few months and I can see a difference in some wrinkles smoothing out, and the age/sun spots lightening... I will continue to apply the lotions over the next year and monitor results.
Thanks to everyone for sharing their Rap experiences and insights.
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Peregrinatum Thats great to hear, thanks for sharing your experience. I too can feel a noticeable difference when I take my weekly dose. But I hear from many that feel absolutely nothing, that is very shocking to me. Let me ask you this, do you also feel something when taking a round of senolytics? I respond to those also yet alot of people say they feel nothing from those also. I took a break like you mentioned and definitely noticed a difference. I will be taking this for life for sure. I am so glad I decided to try it.
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Fred Cloud I have never taken senolytics outside of quercetin, and it didn't give me the same effect as Rap does... It could be that people who experience no/little benefit are not taking enough Rap and not over a long enough period of time... I followed the procedure outline by Van (from the Blagosklonny papers) to find the Goldilocks dose for me... Blogosklonny believes that any unwanted side effects from these low weekly doses (up to 0.2 mg/kg) are reversible... that is what I found in my case as well. Glad to hear Rap is working well for you, I have tried many different therapies/supplements/diets/exercise to feel better during ageing... nothing has come close to the benefits of Rap.
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Peregrinatum I wanted to take a moment and update everyone on the latest from Dr. Blagosklonny and Dr. Green concerning Rapa and other drugs and treatments.
Dr. B is out with his latest recommendations about the drugs that actually work and those that do not for longevity purposes. For the first time he has actually named those that do not work, or lack of evidence. See Life Extension PDF attached. The following cite is from Dr. Green's website dealing with Senolytics to clear out "zombie cells" as recommended by Dr. B in his latest paper. Now he is recommending to take only Fistein 2000 mg and Dasatinib 100 mg. Every 2 weeks, 2 consecutive days once a month, and for younger people once/twice every 3 months. https://senolyticstreatment.com/ No other Senolytics were recommended at this time.
Many people have asked how to measure if Rapa and all of the other treatments are working. It is quite simple, take a blood/urine test measuring certain things in your blood and urine and then taking the appropriate treatment. I will post my own personal tests I use to measure my progress on reducing weight, insulin resistance, CR, reducing inflammation and plaque in vital organs, etc. in the next day or two. Also, you can use the Longevity Advantage calculator(https://www.longevityadvantage.com/mortality-score-and-phenotypic-age-calculator/ to determine what your biological age is vs chronological age. This is very accurate and valid and has been proven in the following paper Phenotypic Age thru many 1,000's of people. Do you have the blood profile of a young person or not and how young. It list all of the normal blood tests that you can have your doctor to prescribe on your next visit or pay for yourself. As always open for discussion.
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Van
[13:42, 2/4/2021] Van: MICROALBUMIN/CREATINE RATIO URINALYSIS = KIDNEY HEALTH
HERE ARE THE BLOOD TEST I RECOMMEND, IF YOU GET THESE 9 TESTS YOU CAN PLUG THEM INTO THE PHENOTYPE FORMULA TO GET YOUR BIOLOGICAL AGE VS CHRONICAL AGE. THESE PHENOTYPE TEST ARE VERY ACCURATE AND HAVE BEEN PROVEN IN MANY TRIALS. IT ALSO TELLS YOU WHAT KIND OF PROGRESS YOUR MAKING ON YOU LONGEVITY JOURNEY.
AlbumiN
Creatinine
Glucose
C-reac Protein
Lympocyte
Mean Cell Volume
Red Cell Dist Width
Alkaline Phosphatase
White Blood CellsREGULAR BLOOD TEST
FASTING GLUCOSE
HbA1c
LIVER ENZYMES
LIPIDS
CBC
PSA
ALL OF THESE TEST ARE REGULAR BLOOD TEST THAT ARE USED EVERYDAY. YOU SHOULD HAVE NO PROBLEM GETTING DOCTOR TO WRITE AN ORDER.I take the following at this moment. Rapa 10 mg. weekly, Metformin 2,000 mg. daily,(glucose control) Crestor 5 mg daily,(lower inflammation throughout the body) Linsopril 10 mg. daily,(lower B/P) B12 1000 mcg daily, B Complex vitamin, K2 daily,(these are to increase production of red blood cells for stamina) Doxazosina Neo 4 mg. x twice daily, Desmopressin .2mg. once daily (both for BPH symptoms), Dasatinib 100 mg + 2000 mg Fisetin one round every 2 weeks. (clear out senescent cells.) Almost everything here can be measured in blood/urine test to find out if you even need the drugs and how much you need. I have decreased my use of linsopril by half due to loss of weight. When I get a chance I will drop or reduce the dosage of drugs.
CIMT to measure cartoid artery plaque, yearly. CT/Angiogram every 5 years to directly measure plaque in the heart muscle and the arteries that feed it. If you have below average plaque in carotid, heart muscle, and kidneys then do not need a statin or Metformin which both control inflammation which cause plaque build up in all these organs and throughout the body. -
Van Thanks for posting this. btw, what do you mean "every 2 weeks... once a month" which is it, every 2 weeks or once a month?
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Fred Cloud Fred Cloud If you read Dr Greens 1st paragraph, the schedule is dependent on age. At 79 yo, Dr Green does ever 2 weeks, maybe at 65 yo, you do every month, every 3 months if young. The zombie cells build up faster the older you are.
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Van where are you sourcing the Fistein and Dasatinib? I tried alldaychemist and that was a no go...
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Peregrinatum Fistein is easy to get online. Also, best and cheapest place to buy it. Powder is best. Make sure it is 99% Fistein, others are selling the 50%. Just weigh out 2,000 mg. Dasatinib is more difficult. I ordered my from China. It is only 100 mg per dose, so 1 gram = 10 doses. Order 5-10 grams powder. This little bit of powder is easy to get thru Customs. Order form company on Alibaba. Alibaba guarantees, you will get what you pay for. COA should come with it.
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Van Thanks brother... any special instructions on taking the powder dosages, such as with fat or with food?
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Peregrinatum I have added Vitamin C, 1,000 mg for bioavailability. Dr. Green and others have recommended it.
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This is a new protocol of NAD replacement therapy especially for people over 65 yo. I believe this one will really work and is not expensive to try. 2-4 weeks, and should feel better, and have more endurance. How does this relate to Rapa? Well Rapa slows down NAD degeneration, but cannot replace it. I feel very good about the science behind this one. Dr. Green's senescence protocol is different, and should still be followed. Clear out those zombie's and replace NAD for increased well being and endurance. Only time will tell, but I will start tomorrow on mine and will give feedback. See Post #1739 https://www.longecity.org/forum/topic/94224-manipulating-mitochondrial-dynamics/page-58
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Van Thanks for posting. I have been looking at this for a while but never tried it. I dont know who that Turnbuckle guy is but he is on a different level with what he comes up with. Here is the direct link to the post.
https://www.longecity.org/forum/topic/94224-manipulating-mitochondrial-dynamics/page-58#entry903440
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Fred Cloud If you are over 60 you probably have noticed a drop in energy, endurance and fatigue. It is an treatment to increase NAD levels lost in aging, but not a rip off like Chromadex, Sinclair and others. In my mind, his protocol is worth trying and does not cost that much. Should see results in 2-4 weeks.
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Greetings folks, very occasional and intermittent lurker, first time poster.
I finally decided to pull the trigger on rapamycin powder, and now when I go to Alibaba there are zero hits for it or sirolimus. Wondering if it was a US thing, I opened a new private browser window and set my shipping destination to Mexico. Still no luck. Anyone else seen/is aware of this and no the reason? Is it still possible to source rapamycin powder?
Suggestions welcome. Thanks!
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Liam_Cohen Are you going to Alibaba.com
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Van Search for Sirolimus.
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Van Yes, Alibaba.com. Zero hits for rapamycin, sirolimus, or even ivermectin. They are all in the auto-complete of the search bar, but come up empty like the results are being filtered.
Also, L-citrullene and aspirin get hits, so it's not like the entire site is acting down. My guess is it's filtering on US-based IP addresses.
Have you checked? You're in Spain?
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Van Yep, comes up no matches. I even changed ship-to country to Spain. No luck. Gotta be filtering on country-level IP addresses.
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Liam_Cohen
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Van Did you hit the link I sent you? The link shows shipping to US., and search rapamycin. Also try a VPN
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Van Yes, that screen cap is what comes up when I click on it.
If they show they'll ship to US, it must be Alibaba filtering results. I bet you're right, a VPN would work. Thx.
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Liam_Cohen I get the same screen you do.
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chuck stanley VPN did the trick, except when I go to Wuhan Hengheda Pharm Co., Ltd. I can't find they have sirolimus. Maybe don't stock it anymore. I'd be so happy to buy from Tailor Made Compounding, but d@mn gatekeepers.
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Liam_Cohen AlldayChemist has Sirolimus in stock. But only in tablet form, unfortunately.
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Liam_Cohen
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chuck stanley Liam_Cohen I reached out to my sales person at Wuhan Hengheda Pharma. Indeed, it is the platform that is restricting access. Whether this is the CPC trying to punish Mr. Ma, or responding to Western government pressure is unclear. I strongly suspect the latter. The company is not “allowed” to list it. In any event, they still have Rapamycin. I ordered another 10g at $42 US per gram. It was quite unwieldy but it was possible and it will be shipped within 15 days. As always, it’s possible it will be apprehended by my government... because they need to protect their Big Pharma constituents. I fear the window is closing. I’ll let you know if this order makes it through. Hopefully the shelf-life in a sealed bag in my freezer will be significantly longer than the stated 2 years. You might also search the misspelled “sirolimusa”... the Chinese sellers, ever resourceful, trying workarounds. I get a couple of suppliers with this approach.
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Van beautiful photo.Liam_Cohen yes, it seems that all patented Pharma products are blocked... Canada too. This strongly suggests that the CPC is responding to western government pressure/concerns. So much for “free enterprise”😜
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Liam_Cohen I played around a bit more. It seems that the Alibaba blockade is pretty specific for rapamycin. Other anti-neoplastics and immune modulators are still up (at least with searches from Canada). I know one person who had a shipment of rapa apprehended and tested (pure rapamycin)... and Health Canada was involved. Shipped by WHP... which may be why they in particular do not come up. You can try initiating a discussion with any of their sales people... I bet they will sell and ship. As before, I'm sure many suppliers are solid... but I don't know them...
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Steve Roedde Thanks for all the leg work. Looks like I need to DM with the company.
Pretty funny that that's one way to have your order assayed.
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Liam_Cohen Yes. Pretty hilarious. Unfortunately he got the assay... but not the product:-). Apparently it was US pressure that shut things down on AliBaba. Ever the bastion of free enterprise... unless....
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Steve Roedde US Congress absolutely believes in free enterprise... whichever lobbyist pays the most wins.
Anyway, I didn't want to jinx myself so didn't want to say anything until my order arrived, which it did today... 10 g from Wuhan Hengheda via Alibaba. I used the built-in VPN with Opera browser. I had to contact them via the alibaba msg service and asked for it directly by name.
I ordered on May 10, it shipped on May 17, arrived in US May 24, and then sat in the USPS for "approval." I contacted the sales rep I was working with on June 2 asking if something was wrong and should I do anything. They said, this is normal, just be patient.
So there you go. Hope this helps folks.
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Liam_Cohen , thanks for the update. Clearly the WHP is the least expensive source out there! Indeed, accurate comments about who is the horse, and who is the rider when it comes to Pharma policy. Are you going to have any assay done (although via blood levels (x2 and spectroscopy (x one by Canada customs), I am increasingly comfortable with them. Good tip about the VPN on the Opera browser... I may try that. I have an email address for the contact I use if folks can’t access via any website... shoot me a message.
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Steve Roedde I didn't get the screaming good deal you've mentioned, but it was certainly cheaper than what I previously thought possible. Cheap enough to take a flyer on 10g.
I'm not sure about an assay. Like you, with all the other confirmations I think risk of fraud is exceedingly low. I have thought about starting with skin cream and if no adverse reaction go to oral ingestion. I might also do a blood test to confirm potency and that I've cut it and dosed properly.
Also, I saw just today that Blagosklonny has been doing 10mg/wk and is considering going to 20mg/10-12 days. Cutting to 10mg dose is soooo much easier than a 2-4mg dose, I'm inclined to take it stronger for that reason as well.
https://twitter.com/Blagosklonny/status/1403520633514905614Again, thanks for sharing. I'd have not done this without reading and feeling encouraged with your experience.
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Liam_Cohen I found that 10mg/week plus 1/4 grapefruit is about max for me. When I went to 10 mg plus 1/3 grapefruit, canker sores developed on my tongue.
Thats not a very precise dosage given the variation in grapefruits but it seems I am pretty close and I do love red grapefruit.
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chuck stanley My understanding is grapefruit has a component which binds to an enzyme that metabolizes rapamycin in the gut. By consuming grapefruit you are effectively taking a 30-80 mg dose as far as the amount getting into your blood stream.
Grapefruit makes a lot of sense if you are paying Big Pharma prices. If you are getting it in powder, it's probably not worth the extra effort, unless you really like grapefruit of course. All the dosage information is derived from the experience and assumption a certain amount of it is being metabolized in the gut and not reaching the bloodstream.
The correct cost-don't-matter solution is getting blood work done and fine-tuning one's dose based upon that. But even then, we don't know what serum levels "should be," we just have a bunch of anecdotal reports from people taking as much as they can until they get mouth sores and backing off a little from there. It's a pretty sad state of science as far as I can tell. It's much more like alchemy, in fact.
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Liam_Cohen
Thanks for making those important points. Your estimate that I was taking a 30-80mg effective dose seemed high, though, so I did the arithmetic.
It’s been known that consuming 8 oz of grapefruit juice increases sirolimus levels by 350% , or 44% increase per 1 oz of juice. Since 1 grapefruit = 5 to 6 oz of juice, my 1/3 grapefruit gave me as much as 2 oz of juice. Therefore the effective dose was about 88% increase, giving me an effective dose of around 19 mg. Along with some sirolimus still in my system from the previous week, it must have been around 20mg that proved to be too much. I’ve taken 14 mg before without any side effects. So I think ¼ grapefruit would be about right. But I’ll be more precise in the future.
I do want the optimal dose, and as one who pays big pharma prices, I have to admit that price is a consideration.
I’m sure everyone has seen this from Blagosklonny:
“The optimal anti-aging dose is a personalized maximum dose that does not cause side effects in a particular patient
Optimal dose of rapamycin for maximal net benefits. Life extension by rapamycin is dose-dependent in rodents. The higher the dose, the higher the anti-aging benefits, including cancer prevention and life extension. In humans, side effects are dose-dependent and net benefits could potentially decrease at very high doses. This point of the highest net benefit is the optimal dose. The optimal dose varies in different individuals due to the variability of potential side effects. Thus, the optimal dose in a particular individual is determined by the emergence of side effects. The treatment can be viewed as life-long phase I/II clinical trial.
In conclusion, the side effects of rapamycin are well-known and reversible. When used on an anti-aging schedule, side effects may be absent but, if not, they may be mitigated by combining rapamycin with other anti-aging drugs (metformin, statins) or by temporarily discontinuing it.
Noteworthy, the alternative to the reversible (and avoidable) side effects of rapamycin/everolimus are the irreversible (and inevitable) effects of aging. And by living longer, our generation will benefit from future anti-aging discoveries”
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chuck stanley Thank you for putting some numbers down and showing the math for all of us. I was just winging it directionally correct, based on a loose recollection. :)
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Van Birthplace of Rapamycin
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Accenture Biotech Co.,Ltd
Tel : 86-029-86230510
Add: Taoyuan North Road 355 ,Lianhu District ,Xi’an
Web: http://www.nmn-powder.comI received 4 g from these guys about a month ago. Trusting them on the purity.
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Patient 139 Today, 5/8 Accenture sent a solicitation email. I guess they are still shipping.
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Is this a legitimate source of Rapamycin and Dasatinib?
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Chuck Frasher
The Antiaging Store has been "out of stock" of their RapaPro for many months. I'm not sure that they are still in business.
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JGC Thank you
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Hi Chuck, Etc.
Yes, These are exactly the guys I got my Rapa and Dasatinib from. And metformin.
Recommended vendor.
Kind and respectful regards,
Rudi Hoffman
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Thank you
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Looks like big pharma is shutting down Alibaba for sourcing our meds... the vendors their will still ship but the transaction can't be done through Alibaba, I rushed in an order for 5 years worth of Rapa and hope it keeps in the freezer Lol
Update: I think my last post 3 months ago or so I reported that the weekly Rapa was giving me high blood pressure 157/90... I did some research and found that some do experience this but within 6 months to a year BP levels return to normal for most... I was at the doctors office a few weeks ago to try and get a covid mask waiver for work, and my blood pressure was 118/75, that is the lowest reading I can ever remember! So looks like the BP is fine now...
thank God for the blessing of Rapamycin. Carry on peeps :)
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Check this site: https://www.made-in-china.com. My only chinese rapa provider, Wuhan Hengheda Pharm Co., sells it no more at Alibaba, but does sell it in this new site for 110$/gram, providing door to door shipping. Surprisingly, if I want to buy rapa from that very same provider, to pay for it they give me a link @Alibaba 😊. BTW, I am european.
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Engadin Zermatt Thanks for the site. My supplier as well. I did reorder a couple of weeks back via Alibaba, but that might not be possible moving forward.
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Engadin Zermatt Engadin Zermatt
I wonder if you have a special contact person at Wuhan Hengheda Pharm Co? Or do you use the company email?
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Staffan Olsson Yes, Sanny Zhang. And I have this message from them since April this year:
"Hi, this is Sanny from Wuhan HHD Pharm, manufacturer of nootropics, anti hairloss, sports nutrition and herbal extracts.
Our shop might be closed by Alibaba since May,2021, our business will be transferred to other web, in case you have any products interested that would like to inquiry from us in the future, pls contact email/skype: hhdpharm05@outlook.com, thank you.whatsapp: +86-13129973353"
If you want to mention me as a reference I am Gonzalo Yebenes.
I guess the new web she mention is made-in-china.com.
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Steve Roedde
At the moment I don’t use rapamycin but will try o buy rapamycin powder from China and I would be grateful to hear how other people make sure they ingest the right amount powder.
I would like to avoid dealing with the powder in mg doses. This even if I have the option of using a mg scale. I would like to find a better way to take the right amount without dealing with a scale every single time.
I see that rapamycin is sold in ready made solutions with DMSO. That makes me think that one way of administering rapamycin powder bought from China is to make your own DMSO solution. Any ideas about this and if it is a useful approach. I am concerned about the stability in DMSO oand how long it can be stored in DMSO?
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Staffan Olsson It’s pretty simple. You mix 1 gram of Rapamycin with one kilo of lactose (I posted instructions somewhere on this site). You do indeed need a mg scale to weigh the Rapamycin.. to make sure it’s exactly one gram... a kitchen scale probably close enough for the lactose. This will give you 1 mg of Rapamycin in one GRAM of lactose. Then weigh the weekly dose you want to take. Measure the volume (5 mg likely ~ a tsp). Then just measure the volume. If you get 5.5 mg one weeknand 4.5 mg the next, it probably doesn’t matter! That’s the way I do it.
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Staffan Olsson Steve Roedde I used chat feature on Alibaba. Whoever they have answer the main chat immediately routed my query another person that negotiated my order, after I stated what I was looking to source.
As for mixing it down, I've been surprised no one has gone with solutions. I'm concerned about getting a homogeneous mixture putting 1 gram of fine powder into 1 kilogram of another fine powder. Rapa is water insoluable, but it dissolves in both DSMO and ethanol. With a solution, there's no mess and hassle filling capsule and finely adjusting one's dose is trivial too.
My plan is to mix 1.5 g into 750mL Everclear. That will give me 2mg/mL or 10mg/tsp. It hadn't occurred to me before now that the rapamycin molecule might decompose in solution, but now that I think about it, I suppose that's a possibility. If anyone knows if that is true, please let me know.
Again, I'm surprised solutions are not the most common approach. Is there a reason besides pills are what doctors, pharmacists, and customers are most used to dealing with... if you're dealing with the general public pills are preferable because people are less likely to mess up the measurement.
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Steve Roedde Probably a silly question, but what if you are lactose-intolerant?
Also, to clarify, you stated that you mix 1 gram of rapamycin with 1 *kilogram* (2.2lbs) of lactose?
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Keith Abe you can use another filler (dextrose for example), if lactose is really a problem, although 5 grams probably won’t be an issue. You could mix it at 2:1, 1000 mg (1 gram) of rapamycin, in 500 grams (1/2 kg) of lactose (or dextrose), giving you 2 mg Rapamycin per 1 gram of lactose.
The final dose at 5 mg per week would then be around a half tsp.
As it turns out, I had to mix another batch, and only had dextrose around. It worked fine, but is awfully sweet (the rapa is pretty tasteless). I made it 2:1 to refuse the glucose load.
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Steve Roedde Thanks for the reply, Steve. I admit that I am having a difficult time imagining how I can add 1 gram of rapamycin (or any powder) with 500 grams of another powder, and have the smaller amount of substance x integrate uniformly with the greater amount of substance y. Is there a secret to mixing the two together?
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Keith Abe I posted that elsewhere on the forum. Briefly. You get a stainless steel bowl, mix by hand, for 10 minutes. 1 gram of Rapamycin with 10 grams of lactose. Then you add, say, 50 grams of lactose, another ten minutes, then 100 grams of lactose, then the rest of the lactose. The early dilutions are most critical to ensure even mixing. You can put the whole lot in two ziplocked bags at the end, and do more mixing while watching a video or something. Make sure the humidity is low.
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Steve Roedde Thank you!
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Steve Roedde I have had experience mixing rapa and lactose powders with good results. Here is a video showing the correct way to do it.
https://www.youtube.com/watch?v=k-3DoyKy0zE They are using a mortar and pestle, but a SS spoon and large bowl work very well. It takes time (2.5 hours for me to mix 1.5 grams rapa with 500 grams of lactose) You are basically doubling the mixture in bowl in each cycle mixing well between cycles. I now have a 2-3 yr supply. I store my rapa mixture in a glass container in the freezer and take out appx. 3 months supply in a small container for the fridge. 500 grams of lactose was really too much, 250 would have been enough in order to weigh accurately. I weigh mixture with a jewelers scale (1/1000 accuracy) from Amazon that cost around $30. Plenty accurate for this. 500,000 mg/1500 mg = 333.333 mg of mixture to equal 1 mg rapa. So 5 mg rapa dose = 5 x 333.333 = 1,665 mg. or 1.65 grams of mixture to weigh on scale. Weigh in a small plastic container, tip it up into mouth and wash down with a little water. No problem.
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Van useful. Similar method. Because I found weighing it every day a POA, having a larger proportion of lactose (or whatever), convenient because the volume is easier to measure with a spoon, I did it that way.
When I started I was taking the rapa daily (it’s for treatment not longevity). I’d weigh the daily dose3-4 times, see what the volume was and consume that. My trough levels were always the same confirming adequate mixing as well as accurate enough dosing.
With weekly dosing, weighing would not be an inconvenience.
The jewellers scale is of course critical for the initial rapa weight. I had ordered 1 gram ... but was sent over 3.4 grams of rapa.
I was more descriptive of the with my other post on compounding ... but it could be anywhere on this site. I thought it was on this thread, but apparently not.
I just knock back the dry powder too. One great mind (you) and I... think alike😉.
Make a great day.
Steve
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Steve Roedde I also might mention, I use the grapefruit juice protocol to take rapa mixture, and follow the dosing schedule of Dr. Blagosklonny and Green. They are taking 20 mg rapa every 12-14 days to get "spike" of rapa into the brain to get max longevity benefits. The high dose allows the rapa to get past the blood brain barrier into the brain where aging is regulated. I do get sores on mouth/tongue when I increase my rapa dose to 8 mg., but anything below that zero side-effects. Been on rapa for 4 years. This GFJ protocol increases dosage to 300-350%. I purposely wanted to check if I had actually received rapa from China or not. There is Sirolimus in my mixture for sure. If you think about the high dose, it is not much different than taking 7-10 mg weekly which many do.
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Steve Roedde Inositol works as well. Many years ago when I had a health food store various people would purchase it to cut various illegal substances with
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Well, no reason other than it just seemed simpler for me... Your concern about stability is reasonable. You might have to do some reading... or discuss with a pharmacist. I am a simple man.. who prefers simple solutions... and powder to powder seemed simple...
J Biomol Screen
2003 Apr;8(2):205-9.doi: 10.1177/1087057103252617.
The effect of room-temperature storage on the stability of compounds in DMSO
Barbara A Kozikowski 1 , Thomas M Burt, Debra A Tirey, Lisa E Williams, Barbara R Kuzmak, David T Stanton, Kenneth L Morand, Sandra L Nelson
- PMID: 12844442
- DOI: 10.1177/1087057103252617
Abstract
The stability of approximately 7200 compounds stored as 20-mM DMSO solutions under ambient conditions was monitored for 1 year. Compound integrity was measured by flow injection analysis using positive and negative electrospray ionization mass spectrometry. Each sample was assessed at the beginning of the study, after 12 months of storage, and at a randomized time point between the initial and final time points of the study. The relationship between length of storage and the probability of observing the compound was described by a repeated-measures logistic regression model. The probability of observing the compound was 92% after 3 months of storage at room temperature, 83% after 6 months, and 52% after 1 year in DMSO. An acceptable limit for compound loss and corresponding maximum storage time for samples in DMSO can be determined based on these results.
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Steve Roedde I see it's listed for sale in a solution with DMSO. So, probably doesn't degrade immediately.
https://www.sigmaaldrich.com/US/en/product/sigma/r8781
In any case, moot for me at least for the next year since I mixed 1 gram into some potato starch I had handy. I made it so 1/4 tsp is 10 mg rapamycin. I plan to take that weekly to start and may adjust if I feel the need.
I have one lingering issue with my knee and elbow joints I'd like to see resolve. If that happens I'd be quite pleased.
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Liam_Cohen I see this "Unless otherwise indicated, solutions are probably best prepared fresh and protected from light."
R0395pis.DOC (sigmaaldrich.com)
It would suit me to have rapamycin in DMSO solution. I will look for further information about storage and degrading.
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So far, my insurance is covering my (5mg/week) sirolimus costs, so I am paying close to nothing.
