Resveratrol -- value, dose, source?

David Hanson This company is also selling Fisetin in 500 mg capsules. I have never bought any of their products but I am glad that someone is bringing 500 mg capsules of Fisetin to market. 

https://store.revgenetics.com/collections/all-products/products/senolytics-500-fisetin-500mg

Staffan Olsson 

Careful!  Their caps are 250 mg, NOT 500 mg.  For some reason, these pill peddlers misrepresent their product by putting the "serving size" (500 mg) on the label instead of the capsule content (250 mg).  I really object to this deceptive practice.

JGC Thank you for bringing many attention to this. So obvious that it is deceptive. Now when I look at the pictures, non of them show the full label. 

Laurence R I strongly disagree that all sources are the same "except expensive pharmaceutical grades". Look at testing for supplements and you will find that it is appalling what is put in some of these items - up to 80% don't have or match what is in their labels. Some companies are trying to do it right. Go with brands that have strong reputations in biohacking/life extension circles and or third party testing plus GMP seals (good manufactuing process regulated by fda).

Otherwise, you may be throwing your money to the wind (not matter how cheap, if it's not what it says it is or is degraded it's obviously not going to work). There are only 4-5 companies I will order from right now...each person has to have their own comfort level, but I would rather pay a bit more to know that what I am getting is what I am paying for.

BrianMDelaney He also mentions that a lean body and a fatty one react differently to resveratrol, that there may be an optimal moment of the day to take it, and that to take the wrong amount and at the wrong time may even be counterproductive.
https://youtu.be/IhPnpIcHXB4?t=2512 (shortcut to the point on resveratrol. The entire interview is most interesting with lots of info).

Quote:
"... the body can even get used to hormesis, and you want to be changing things up in your daily life, probably in supplements... we have this standard model that if something works, if you take it 3 times a day, it'll work even better. And, that's not true. When you take it, how much you take, in terms of the day, and whether you exercise, whether you've eaten... All these things play and it's extremely complicated... And if anyone says they know the answer, they are lying..."
 

JGC Have you looked at the bioavailability of micronized resveratrol?  Revgenetics claims that their micronized resveratrol is up to 1000% more bioavailable.

https://store.revgenetics.com/collections/all/products/m98-bulk-micronized-resveratrol

David Hanson 

Interesting.  That chart looks promising, in that they achieve 200 micromoles per milliliter at the peak.  However, it lasts for only about 20 minutes.  That's indeed a x100 improvement over just taking the other supplement pills.  But apparently one has to take the micronized resveratrol with the Tween 80 emulsifier.  That's not what your link is selling, but apparently it is available in their "NitroResvaratrol" liquid capsules.  My main concern, then, is that a 20 minute burst in the bloodstream may not stay around long enough to do much.  I think that in the cell culture studies there was not such a short exposure time.

JGC Do plasma levels correspond with intracellular levels I wonder?

JGC

David H

200Micromole?  how many capsules? also is this in humans or mice(glimpsed the patent and some of it talks about mice.  IIRC, humans metabolize things slower, so that would be relevant.)?

The following paper seems to use a 5 micromole solution for 24hr and it achieved excellent results.

https://bmcmolcellbiol.biomedcentral.com/articles/10.1186/s12860-017-0147-7

In the following paper 25 Micromole appears to induce htert with a peak at 6hours, and it seems not to be too different from 50Micromole in its effect.  Also even at just 3 hours there appears to be significant effect.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4484421/

Question is how would 5 Micromole compare to 25 Micromole?  And how would it differ between plasma vs in vitro solution.   Because it seems a few hours at 5Micromole plasma should be easily achievable if the graph is true(assuming few or one capsule).

Darian S  Can't seem to edit:   For reference in another species

prolonged 100uM rather than lengthen shortens lifespan in yeast, iirc. 10uM lengthens lifespan of yeast.

The graph shows 200micromole is only maintained for a short while.   But that seems to be note for potential caution with further bioavailability enhancements.   We don't know how humans will behave if such high levels were to be sustained.

https://www.biorxiv.org/content/10.1101/2020.07.17.209411v1.full.pdf

Dan Nave 

OK, I think I have the Resveratrol situation sorted out, and I plan to proceed as follows:

I just ordered 2 bottles of Nitro Super Micronized Resveratrol (Nitro250) caps (30 x 250 mg) from RevGenetics ($37.95/bottle). These nitrogen-filled caps contain Resveratrol in micron-size particles suspended in the Tween 80 surfactant emulsifier.  This is the combo that shows the big spike on the chart David Hanson supplied.

To overcome the short Resveratrol residence duration in the bloodstream (~20 minutes) that the chart indicates, I plan to take the caps along with BioPerine, a piperine supplement that I already have on hand and have been using in combination with semiannual megadoses of Fisetin and D+Q.  Piperine is known to suppress the production of P-glycoprotein,  a protein that the body uses to break down exogenous compounds found in the body and that is responsible for the short half-lives of polyphenols in the blood stream.

I think this strategy will yield the maximum bioavailability of Resveratrol.  I am inclined to do this weekly rather than daily (comments?).  I will report any subjective observations of results, once the caps arrive.