Dr. Sinclair claims he reversed his Horvath clock by 29 years!
Go to 8:45 in this video. Said his biological clock was 60 and he reduced it to 31 by changing his life style.
Either he is lying or he is doing something he is not telling us.
I just watched the video. David Sinclair did NOT say that he had reprogrammed his own epigenetic clock from 60 to 31 years. That was his example of what might be accomplished sometime in the future. Further, Sinclair himself is only 50, not 60.
What he did accomplish was to use a CRISPR-modified AAV (adeno-associated virus) to implant the DNA coding for three of the Yamanaka factors into the eye cells of old mice with failing vision. This restored the deteriorated vision of the old mice and reset the epigenetic clocks of their eye cells. The eye is a relatively closed system, so they could restrict the AAV range by direct injection into the eyeball. Sinclair said that his lab is presently attempting to do such AAV-delivered reprogramming for the whole mouse.
The AAV virus technique used by Sinclair is an interesting delivery vehicle for putting DNA and Yamanaka factors where you want them The AAV virus can deliver DNA to all cells that it encounters, or it can be modified to seek out specific cell-types like liver or skin cells. But the problem with this approach, as I see it is that there is no OFF switch. The cell's natural defense mechanism will ultimately find the virus DNA and remove it, but it is not clear how long that takes. Thus, the cell will keep cranking out Yamanaka factors to do more and more reprogramming over a time period that is poorly-defined and possibly quite long. Too much Yamanaka reprogramming makes all cell-types into embryonic stem cells.
I think a better delivery technique is that developed by Turn Bio, in which messenger RNA coded for the Yamanaka factors (and a few other proteins) is delivered by a hypo-allergenic liposome. The mRNA is guaranteed to be transcribed by cell ribosomes only a few times before dissipating, so the epigenetic reprogramming can be done in bursts that are more precisely controlled (e.g., once a day for three days). At present, the Turn liposomes deliver mRNA to all cells reached by the circulatory system, but there is some promise that targeted liposomes can be developed to seek out particular cell types.
David Sinclair has not done a Horvath clock test yet, in an interview he was asked about this question and he said he had not done one.
What he did do is using a company called Inside Tracker (which he is an investor). Inside Tracker uses blood test result to estimate biology age. In the following video, he said his biology age went up to 58, then after taking NMN, dropped to 32.
scroll to 36:09 time mark.