I am 87 years old. I have been doing some form of 3-day D+Q senolytic sessions about every 6 months for the past several years. Before starting, I had several colonoscopies in which 5-10 pre-cancerous polyps were removed. My last colonoscopy turned up only a single millimeter-size polyp, and I attribute this change to the senolytics.
Also, my skin is less papery and my face and neck are smoother. My energy level is good. I do competitive dog agility with my two Shetland Sheepdogs. In weekend trials we tend to do better than almost all of our 20-50 year old competitors, qualifying in at least half of our runs in every trial.
In the last few senolytic sessions I have added Fisetin to D+Q, and in the future I intend to add Ginger Root Extract (see my post), which in human fibroblast skin cultures has been shown to be a bit more effective then D+Q in clearing senescent cells.
So I took another dose of D & Q at the 2.5mg/kg D and 20mg/kg Q and again I see an improvement to my face. I honestly can’t pinpoint what the difference is but it’s noticeable. That being said if I take a photo I can’t see the improvement from before and after. I’m not sure if it’s skin clarity or thickness or tightness but it’s definitely not my imagination. I plan to take another dose tomorrow (a week after initial dose). It may require a few months of doses to get good photographic evidence.
hi guys im new just signed up ... im a 72 year old physicist but have been studying human biochemistry since i was 26 (specifically the ageing process). now to business... My understanding in the D+Q experiments the time to rejuvenation (after dosing) was 2 months. Considering that normal lifespan is 24 months and assuming human and mouse responses are equal (which they certainly are not) would hint at equivalent human rejuvenation at approximately 6 years or 1/12 of our lifespan. in other words...we are probably not there yet. Whether or not, along the way, we are capable of seeing intermediate results is questionable and likely subjective. This is the essence of what bio- researchers call anecdotal evidence.
Please do not misunderstand, I am arguing for Patience, not against experimentation. I too have extensively dabbled in senolytics since the day I read kirkland's seminal paper that immediately changed everything I had learned about ageing from the "old days". I must say I have been impressed by the quality of some of the experiments I have read here. So what, is everybody trained in laboratory technique now days? Just kidding.
So here's what i see:
1 many researchers, as well as some on this site, have argued for an objective measure of progress toward rejuvenation. Kirkland himself in a followup paper speaks to the difficulty of achieving this because of what he calls, the large variety of different senescent cells that respond differently to various attacks. That said it is clear to me much of senescence is directed by the mitochondria (perhaps partially controlled by the nucleus). Therefore much of the research is correctly directed at apoptosis (the natural process of cell death that these "zombie cells" are conspiring to avoid). But even in this particular case, a biochemical test to identify the death of these cells from senolytic use appears elusive. So we are left with what we can share here to try and fathom any progress we are making. To a physicist, who likes to have the world explained in building blocks (from the bottom up), this is akin to "stamp collecting" and then looking for patterns. Still it's what we have, here on the front lines and it is exciting to be at the forefront, (besides, the alternative is to circle the bowl waiting for the next flush). So on this subject, if any one knows of a revealing test, or even a paper on point in identifying senescent cell death, im all ears.
2 Arrogance: We are here engaged in trying to do something that has never been done before....If you are not arrogant you probably don't belong here. My own test for arrogance is the number of times I make the same mistake before i realize that the physical universe is not cooperating with my expectations. (this test does not include any reference to Quantum Theory, which I blame for the disappearance of socks in the dryer). So please feel free to correct me, (cause if I aint learning, i aint growing and if I aint growing i'm dying...and that just ruins the whole experiment).
3 Safety: It is common knowledge on this forum that large intermittent doses of senolytics is the key to rejuvenation. In your preparation for a new experiment be sure to first titrate the dose. Because people are different (biochemical individuality), you can have a bad reaction to anything, so test with a little first. I was pleased to note that one recent contributer was quite salient on this point (could there be a doctor in the house?). Cant remember his name...I remember impressions not names which is why i cant spell. Anyway, to the point, If we were all the same, this forum would not exist because all human problems would be solved already, so be careful.
4 Lisanti, Michael P.
I have not noticed any discussion of this researcher on this forum. He has developed a protocol for the destruction of cancer stem cells. and has thus explained the reason that cancer comes back. The protocol is DAC: Doxycycline, Azithromycin, and vitamin C. He has stated that the antibiotics can be given in sub-antimicrobial doses because of their senolytic properties to "corral" the nasty cancer stem cells, leaving them vulnerable to faulty mitrochondrial regeneration, which is then "pushed" with vitamin C to their eventual destruction. (no more looking over your shoulder for cancer to return.) If you think this is hooey, the med literature is replete with low dose Azithromycin being given to CF patients with terribly scarred nonfunctional lungs to effectively remodel their lung tissue. This protocol is expected to be effective for all damaged lung tissue regardless of source. It is clearly the senolytic ability of Azithromycin that removes the built up inflammatory senescent lung cells allowing replacement by the healing properties of the body. This is akin to the senolytic developed by unity biotechnology that in clinical trials is repairing advanced macular degeneration in people who previously were blind. But back to the point, Dr. lisanti has already run successful clinical trials on human subjects, and even better than that his published papers are easily readable to the interested and can be accessed on google scholar. I highly recommend these as a portal into the entire field of antibiotic senolytics, and as another addition to your bag of tricks.
Its a new world gentlemen....have at it.