Introduction to senolytics

I am just preparing to begin a C+D protocol. I am going to take based on my weight of 180 pounds 200 mg of Dasatinib and 2000 mg of Quercetin. My protocol will be two rounds, one week apart. I will advise of my experience after starting the protocol. There are lengthy accounts of experimentation on a number of longevity sites such as LongCity.org .

I have called two compounding pharmacies looking for dasatinib. But no luck finding the inexpensive source.

A future alternative to the C+D senolytic treatment (which has some concerning side effects) is the DNA approach to clearing senescent cells.  A Seattle-based bio-startup called Oisin Biotechnologies has sequenced a plasmid (a ring of DNA) that is encased in a lipisome (a lipid bubble) for delivery inside cell walls.  The plasmid/lipisome is suffused into the bloodstream and enters most cells.  The plasmid is sequenced to detect whether the cell is senescent, as determined by whether it is generating transcription factors to express the protein p16.  If p16 expression is detected, the plasmid triggers a "suicide gene" that causes the cell to go into aptosis, to neatly disassemble itself, and to disappear.  The same Oisin technique can detect and induce aptosis in malignant cells that are expressing the cancer-signal protein p53.  I wrote a column about this for Analog Magazine, which is available HERE.

The Oisin technique is presently being tested on aging mice (see LINK), and the ongoing tests have so far has shown that with the best treatment the mice become visibly healthier and live longer.  There are no known side effects to this senescent cell clearance, except for possible wound-healing delay and pregnancy complications.  There are currently plans for tests the Oisin technique on other animals and on humans, but it will be some years before the treatment is generally available.

I have purchased Dasatinib through Medimpex, a India phara supply company.  I deal with an individual named Soni-Jan.   I have purchased both Rapamycin and Dasatinib through this company.  Seem to be legitimate however since I have not assayed their products I can't say for sure, however doing searches on the specific products I have purchased they appear to be legitimate.   Now my purchase of Dasatinib was made through MedImpex.  I purchased a 20mg bottle of 60 capsules for a total of 189.00 plus 25 dollars express EMS shipping.  I purchased this last year but have waited to find the results of the recent study.  At the recommenced dose, for my weight, I will have to take 200 mg Dasatinib and 2000 of Quercertin.   My purchase thus yields enough Dasatinib for six dosages.   or two every 6 months.  Below is a photo of the Dasatinib box.  I have been out of the country and plan on start the first week protocol soon.

The following post that I originally placed under "Self-Experimenting/Share" may be a better fit here! "I mentioned the senolytic supplement fisetin under Rapamycin cost and mentioned one of the references. I received my 100mg fisetin caps yesterday ($15/30 Swanson) so commenced a fisetin/quercetin senolytic trial of my own design (not a doc but do have an MS in biochemistry and studied some pharmacology). I tried 1 g (10 caps) of fisetin instead of the dasatinib (based on the planned dosage for an upcoming trial w/ post-menopausal women) along w/ the prescribed ~2g (my weight=165) quercetin recommended in Bill Faloon's RAADFest handout. No noticeable bad side effects since taking at 4pm yesterday and foot pain (likely neuropathic) seems about 30% better along w/ possible increased energy. Planning a repeat dosing in a week as recommended in the Dasatinib/quercetin protocol, but w/ fisetin/quercetin." Fisetin is much easier to get and seems to have fewer side effects, and so far, nice benefit after two years of foot nerve pain.

I also ordered Dasatinib from BonHoa.  Package was opened by customs, resealed, and delivered in about 2 weeks.  So it is legal, or at least acceptable to our guardians.

No matter what senolytic you are taking, it sounds like you should take it in large intermittent doses? I.e. Quercetin is not a once a day supplement like most people probably take it.